ADIPOQ ELISA 试剂盒
(13 references)-
- 抗原 See all ADIPOQ ELISA试剂盒
- ADIPOQ (Adiponectin (ADIPOQ))
- 抗原表位
- AA 18-247
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 312-20000 pg/mL
- 最低检测浓度
- 312 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Adiponectin
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate, Urine
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: E18-N247 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: E18-N247 - Featured
- Discover our best selling ADIPOQ ELISA Kit
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- Discover our top product ADIPOQ ELISA Kit
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Adiponectin (ADIPOQ) ELISA Kit
ADIPOQ 适用: 人 Colorimetric Sandwich ELISA 1.562-100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 绵羊 Colorimetric Competition ELISA 6.25 ng/mL - 100 ng/mL Plasma, Serum
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 小鸡 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 人 AA 19-244 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate, Urine
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 兔 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
-
Sequence similarities: Contains 1 C1q domain.
Tissue Specificity: Synthesized exclusively by adipocytes and secreted into plasma.
- 板类型
- Pre-coated
- 实验流程
- mouse Adiponectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Adiponectin has been precoated onto 96-well plates. Standards(NSO, E18-N247) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Adiponectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Adiponectin amount of sample captured in plate.
- 实验流程
-
Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse Adiponectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Adiponectin standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(ng/ml): 3.35, Standard deviation: 0.160, CV(%): 5
- Sample 2: n=16, Mean(ng/ml): 6.82, Standard deviation: 0.360, CV(%): 5.3
- Sample 3: n=16, Mean(ng/ml): 13.50, Standard deviation: 0.756, CV(%): 5.6,
- Sample 1: n=24, Mean(ng/ml): 3.39, Standard deviation: 0.190, CV(%): 5.4
- Sample 2: n=24, Mean(ng/ml): 6.60, Standard deviation: 0.380, CV(%): 5.8
- Sample 3: n=24, Mean(ng/ml): 13.20, Standard deviation: 0.818, CV(%): 6.2
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
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: "Programming of mouse obesity by maternal exposure to concentrated ambient fine particles." in: Particle and fibre toxicology, Vol. 14, Issue 1, pp. 20, (2017) (PubMed).
: "Oral Administration of Resveratrol Alleviates Osteoarthritis Pathology in C57BL/6J Mice Model Induced by a High-Fat Diet." in: Mediators of inflammation, Vol. 2017, pp. 7659023, (2017) (PubMed).
: "Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE-/- Mice through the ROS/MAPK/NF-κB Pathway." in: Nutrients, Vol. 7, Issue 8, pp. 7085-105, (2016) (PubMed).
: "Puerarin Protects Pancreatic β-Cells in Obese Diabetic Mice via Activation of GLP-1R Signaling." in: Molecular endocrinology (Baltimore, Md.), Vol. 30, Issue 3, pp. 361-71, (2016) (PubMed).
: "Extracted Triterpenes from Antrodia cinnamomea Reduce the Inflammation to Promote the Wound Healing via the STZ Inducing Hyperglycemia-Diabetes Mice Model." in: Frontiers in pharmacology, Vol. 7, pp. 154, (2016) (PubMed).
: "Phylloquinone supplementation improves glycemic status independent of the effects of adiponectin levels in premonopause women with prediabetes: a double-blind randomized controlled clinical trial." in: Journal of diabetes and metabolic disorders, Vol. 14, Issue 1, pp. 1, (2015) (PubMed).
: "Effect of Lactobacillus plantarum Strain K21 on High-Fat Diet-Fed Obese Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 391767, (2015) (PubMed).
: "A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes." in: Natural products and bioprospecting, Vol. 5, Issue 3, pp. 159-66, (2015) (PubMed).
: "Glypican 4 may be involved in the adipose tissue redistribution in high-fat feeding C57BL/6J mice with peroxisome proliferators-activated receptor ? agonist rosiglitazone treatment." in: Experimental and therapeutic medicine, Vol. 8, Issue 6, pp. 1813-1818, (2014) (PubMed).
: "Adiponectin is expressed in the pancreas of high-fat-diet-fed mice and protects pancreatic endothelial function during the development of type 2 diabetes." in: Diabetes & metabolism, Vol. 40, Issue 5, pp. 363-72, (2014) (PubMed).
: "The Effects of 6 Isocaloric Meals on Body Weight, Lipid Profiles, Leptin, and Adiponectin in Overweight Subjects (BMI > 25)." in: International cardiovascular research journal, Vol. 8, Issue 2, pp. 52-6, (2014) (PubMed).
: "Eicosapentaenoic acid-enriched phospholipid ameliorates insulin resistance and lipid metabolism in diet-induced-obese mice." in: Lipids in health and disease, Vol. 12, pp. 109, (2013) (PubMed).
: "Effects of resveratrol on the amelioration of insulin resistance in KKAy mice." in: Canadian journal of physiology and pharmacology, Vol. 90, Issue 2, pp. 237-42, (2012) (PubMed).
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: "Programming of mouse obesity by maternal exposure to concentrated ambient fine particles." in: Particle and fibre toxicology, Vol. 14, Issue 1, pp. 20, (2017) (PubMed).
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- 抗原 See all ADIPOQ ELISA试剂盒
- ADIPOQ (Adiponectin (ADIPOQ))
- 别名
- ADIPOQ (ADIPOQ 产品)
- 别名
- ADP ELISA Kit, DCAF9 ELISA Kit, 6720489L24Rik ELISA Kit, 9330209L04Rik ELISA Kit, Adp ELISA Kit, Mamdc1 ELISA Kit, RNSVSP4U ELISA Kit, SVSIV1 ELISA Kit, Svp4 ELISA Kit, ACDC ELISA Kit, ACRP30 ELISA Kit, ADIPQTL1 ELISA Kit, ADPN ELISA Kit, APM-1 ELISA Kit, APM1 ELISA Kit, GBP28 ELISA Kit, apM-1 ELISA Kit, 30kDa ELISA Kit, APN ELISA Kit, Acdc ELISA Kit, Acrp30 ELISA Kit, Ad ELISA Kit, adipo ELISA Kit, apM1 ELISA Kit, ADN ELISA Kit, acrpl ELISA Kit, adipo-b ELISA Kit, adipoql ELISA Kit, zgc:153584 ELISA Kit, adipoq ELISA Kit, WD and tetratricopeptide repeats 1 ELISA Kit, MAM domain containing glycosylphosphatidylinositol anchor 2 ELISA Kit, seminal vesicle secretory protein 4 ELISA Kit, adiponectin, C1Q and collagen domain containing ELISA Kit, adiponectin, C1Q and collagen domain containing, b ELISA Kit, adiponectin, C1Q and collagen domain containing L homeolog ELISA Kit, WDTC1 ELISA Kit, Mdga2 ELISA Kit, Svs4 ELISA Kit, ADIPOQ ELISA Kit, Adipoq ELISA Kit, adipoqb ELISA Kit, adipoq.L ELISA Kit
- 背景
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Protein Function: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. .
Background: Adiponectin(ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.
Synonyms: Adiponectin,30 kDa adipocyte complement-related protein,Adipocyte complement-related 30 kDa protein,ACRP30,Adipocyte, C1q and collagen domain-containing protein,Adipocyte-specific protein AdipoQ,Adipoq,Acdc, Acrp30, Apm1,
Full Gene Name: Adiponectin
Cellular Localisation: Secreted. - 基因ID
- 11450
- UniProt
- Q60994
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