Resistin ELISA 试剂盒
(2 references)-
- 抗原 See all Resistin (RETN) ELISA试剂盒
- Resistin (RETN)
- 抗原表位
- AA 19-108
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78-5000 pg/mL
- 最低检测浓度
- 78 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Resistin
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: E.coli
Immunogen sequence: K19-P108 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <3pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: E.coli
Immunogen sequence: K19-P108 - Featured
- Discover our best selling RETN ELISA Kit
- Top Product
- Discover our top product RETN ELISA Kit
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Resistin (RETN) ELISA Kit
RETN 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Resistin (RETN) ELISA KitRETN 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Resistin (RETN) ELISA KitRETN 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Resistin (RETN) ELISA KitRETN 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Resistin (RETN) ELISA KitRETN 适用: Cow Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Resistin (RETN) ELISA KitRETN 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Resistin (RETN) ELISA KitRETN 适用: 绵羊 Colorimetric Sandwich ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
Resistin (RETN) ELISA KitRETN 适用: 兔 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Resistin (RETN) ELISA KitRETN 适用: Pig Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Resistin (RETN) ELISA KitRETN 适用: 猴 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the resistin/FIZZ family.
Tissue Specificity: Expressed only in fatty tissues.
- 板类型
- Pre-coated
- 实验流程
- human Resistin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Resistin has been precoated onto 96-well plates. Standards(E.coli, K19-P108) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Resistin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Resistin amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human Resistin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin , EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Resistin standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 534, Standard deviation: 28.84, CV(%): 5.4
- Sample 2: n=16, Mean(pg/ml): 1453, Standard deviation: 88.63, CV(%): 6.1
- Sample 3: n=16, Mean(pg/ml): 2323, Standard deviation: 109.2, CV(%): 4.7,
- Sample 1: n=24, Mean(pg/ml): 554, Standard deviation: 45.98, CV(%): 8.3
- Sample 2: n=24, Mean(pg/ml): 1532, Standard deviation: 119.5, CV(%): 7.8
- Sample 3: n=24, Mean(pg/ml): 2486, Standard deviation: 200, CV(%): 8
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
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: "Obesity, Serum Resistin and Leptin Levels Linked to Coronary Artery Disease." in: Arquivos brasileiros de cardiologia, Vol. 107, Issue 4, pp. 348-353, (2017) (PubMed).
: "Serum resistin positively correlates with serum lipids, but not with insulin resistance, in first-degree relatives of type-2 diabetes patients: an observational study in China." in: Medicine, Vol. 96, Issue 16, pp. e6622, (2017) (PubMed).
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: "Obesity, Serum Resistin and Leptin Levels Linked to Coronary Artery Disease." in: Arquivos brasileiros de cardiologia, Vol. 107, Issue 4, pp. 348-353, (2017) (PubMed).
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- 抗原 See all Resistin (RETN) ELISA试剂盒
- Resistin (RETN)
- 别名
- RETN (RETN 产品)
- 别名
- RETN ELISA Kit, ADSF ELISA Kit, FIZZ3 ELISA Kit, RETN1 ELISA Kit, RSTN ELISA Kit, XCP1 ELISA Kit, Fizz3 ELISA Kit, Rstn ELISA Kit, Xcp4 ELISA Kit, resistin ELISA Kit, RETN ELISA Kit, Retn ELISA Kit
- 背景
-
Protein Function: Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.
Background: Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance.1 Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin(for resistance to insulin), that may be the hormone potentially links obesity to diabetes.2 Resistin is overexpressed in human adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.3 The standard product used in this kit is human Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.
Synonyms: Resistin,Adipose tissue-specific secretory factor,ADSF,C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein,Cysteine-rich secreted protein A12-alpha-like 2,Cysteine-rich secreted protein FIZZ3,RETN,FIZZ3, HXCP1, RSTN,UNQ407/PRO1199,
Full Gene Name: Resistin
Cellular Localisation: Secreted. - 基因ID
- 56729
- UniProt
- Q9HD89
- 途径
- Feeding Behaviour, Smooth Muscle Cell Migration
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