TGFB1 ELISA 试剂盒
(29 references)-
- 抗原 See all TGFB1 ELISA试剂盒
- TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))
- 抗原表位
- AA 279-390
-
适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Mouse TGF beta 1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (EDTA), Urine
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: CHO
Immunogen sequence: A279-S390 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <2pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: CHO
Immunogen sequence: A279-S390 - Featured
- Discover our best selling TGFB1 ELISA Kit
- Top Product
- Discover our top product TGFB1 ELISA Kit
-
Transforming Growth Factor, beta 1 (TGFB1) ELISA Kit
TGFB1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 人 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma, Serum
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 人 AA 279-390 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum, Urine
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 小鸡 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 犬 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 马 Colorimetric Competition ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- mouse TGF beta 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TGF beta 1 has been precoated onto 96-well plates. Standards(CHO, A279-S390) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF beta 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TGF beta1 amount of sample captured in plate.
- 实验流程
-
Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse TGF beta1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TGF beta 1 standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 114, Standard deviation: 5.47, CV(%): 4.8
- Sample 2: n=16, Mean(pg/ml): 240, Standard deviation: 12, CV(%): 5
- Sample 3: n=16, Mean(pg/ml): 483, Standard deviation: 17.9, CV(%): 3.7,
- Sample 1: n=24, Mean(pg/ml): 125, Standard deviation: 6.75, CV(%): 5.4
- Sample 2: n=24, Mean(pg/ml): 248, Standard deviation: 15.4, CV(%): 6.2
- Sample 3: n=24, Mean(pg/ml): 515, Standard deviation: 30.4, CV(%): 5.9
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
-
: "The effects of 1,4-dimethylpyridine in metastatic prostate cancer in mice." in: BMC cancer, Vol. 17, Issue 1, pp. 177, (2018) (PubMed).
: "Oroxylin A inhibits the generation of Tregs in non-small cell lung cancer." in: Oncotarget, Vol. 8, Issue 30, pp. 49395-49408, (2018) (PubMed).
: "Combined Blockade of T Cell Immunoglobulin and Mucin Domain 3 and Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 Results in Durable Therapeutic Efficacy in Mice with Intracranial Gliomas." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 3593-3602, (2018) (PubMed).
: "Epigallocatechin-3-gallate inhibits inflammation and epithelial‑mesenchymal transition through the PI3K/AKT pathway via upregulation of PTEN in asthma." in: International journal of molecular medicine, Vol. 41, Issue 2, pp. 818-828, (2018) (PubMed).
: "Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 2305683, (2018) (PubMed).
: "LincRNA 1700020I14Rik alleviates cell proliferation and fibrosis in diabetic nephropathy via miR-34a-5p/Sirt1/HIF-1α signaling." in: Cell death & disease, Vol. 9, Issue 5, pp. 461, (2018) (PubMed).
: "Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer." in: Oncology letters, Vol. 15, Issue 6, pp. 9293-9298, (2018) (PubMed).
: "Targeting proinflammatory cytokines, oxidative stress, TGF-β1 and STAT-3 by rosuvastatin and ubiquinone to ameliorate trastuzumab cardiotoxicity." in: Biomedicine & pharmacotherapy, Vol. 93, pp. 17-26, (2017) (PubMed).
: "Aberrant low expression of p85α in stromal fibroblasts promotes breast cancer cell metastasis through exosome-mediated paracrine Wnt10b." in: Oncogene, Vol. 36, Issue 33, pp. 4692-4705, (2017) (PubMed).
: "Herbal Compound Songyou Yin and Moderate Swimming Suppress Growth and Metastasis of Liver Cancer by Enhancing Immune Function." in: Integrative cancer therapies, Vol. 15, Issue 3, pp. 368-75, (2017) (PubMed).
: "Toxoplasma gondii Elongation Factor 1-Alpha (TgEF-1α) Is a Novel Vaccine Candidate Antigen against Toxoplasmosis." in: Frontiers in microbiology, Vol. 8, pp. 168, (2017) (PubMed).
: "Effects of Recombinant Toxoplasma gondii Citrate Synthase I on the Cellular Functions of Murine Macrophages In vitro." in: Frontiers in microbiology, Vol. 8, pp. 1376, (2017) (PubMed).
: "Ilomastat, a synthetic inhibitor of MMPs, prevents lung injury induced by γ-ray irradiation in mice." in: Oncotarget, Vol. 8, Issue 37, pp. 60789-60808, (2017) (PubMed).
: "Toxoplasma gondii excretory/secretory antigens (TgESAs) suppress pro-inflammatory cytokine secretion by inhibiting TLR-induced NF-κB activation in LPS-stimulated murine macrophages." in: Oncotarget, Vol. 8, Issue 51, pp. 88351-88359, (2017) (PubMed).
: "Clopidogrel in a combined therapy with anticancer drugs-effect on tumor growth, metastasis, and treatment toxicity: Studies in animal models." in: PLoS ONE, Vol. 12, Issue 12, pp. e0188740, (2017) (PubMed).
: "GHK Peptide Inhibits Bleomycin-Induced Pulmonary Fibrosis in Mice by Suppressing TGFβ1/Smad-Mediated Epithelial-to-Mesenchymal Transition." in: Frontiers in pharmacology, Vol. 8, pp. 904, (2017) (PubMed).
: "Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding Toxoplasma gondii elongation factor 1-alpha." in: BMC infectious diseases, Vol. 15, pp. 448, (2016) (PubMed).
: "A deleterious role for Th9/IL-9 in hepatic fibrogenesis." in: Scientific reports, Vol. 6, pp. 18694, (2016) (PubMed).
: "Mature dendritic cells cause Th17/Treg imbalance by secreting TGF-β1 and IL-6 in the pathogenesis of experimental autoimmune encephalomyelitis." in: Central-European journal of immunology, Vol. 41, Issue 2, pp. 143-52, (2016) (PubMed).
: "Mice overexpressing integrin ?v in fibroblasts exhibit dermal thinning of the skin." in: Journal of dermatological science, Vol. 79, Issue 3, pp. 268-78, (2015) (PubMed).
-
: "The effects of 1,4-dimethylpyridine in metastatic prostate cancer in mice." in: BMC cancer, Vol. 17, Issue 1, pp. 177, (2018) (PubMed).
-
- 抗原 See all TGFB1 ELISA试剂盒
- TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))
- 别名
- TGFB1 (TGFB1 产品)
- 别名
- CED ELISA Kit, DPD1 ELISA Kit, LAP ELISA Kit, TGFB ELISA Kit, TGFbeta ELISA Kit, TGF-beta ELISA Kit, TGF-BETA-1 ELISA Kit, TGF-beta5 ELISA Kit, ced ELISA Kit, dpd1 ELISA Kit, lap ELISA Kit, tgf-beta ELISA Kit, tgfb ELISA Kit, tgfb5 ELISA Kit, tgfbeta ELISA Kit, TGF-beta1 ELISA Kit, TGFbeta1 ELISA Kit, Tgfb ELISA Kit, Tgfb-1 ELISA Kit, ai39657 ELISA Kit, tgfb1 ELISA Kit, wu:fb13a07 ELISA Kit, xx:ai39657 ELISA Kit, TGFB1 ELISA Kit, csd ELISA Kit, cdb1 ELISA Kit, cdg2 ELISA Kit, csd1 ELISA Kit, csd2 ELISA Kit, csd3 ELISA Kit, ebmd ELISA Kit, lcd1 ELISA Kit, bigh3 ELISA Kit, cdgg1 ELISA Kit, betaig-h3 ELISA Kit, TGFB4 ELISA Kit, transforming growth factor beta 1 ELISA Kit, transforming growth factor beta-1 ELISA Kit, transforming growth factor beta 1 L homeolog ELISA Kit, transforming growth factor, beta 1 ELISA Kit, transforming growth factor, beta 1a ELISA Kit, transforming growth factor beta induced L homeolog ELISA Kit, TGFB1 ELISA Kit, Tgfb1 ELISA Kit, tgfb1.L ELISA Kit, tgfb1a ELISA Kit, tgfbi.L ELISA Kit
- 背景
-
Protein Function: Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T- helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells (PubMed:18368049). .
Background: Transforming growth factor-beta1(TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects. TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation. TGFbeta1 is also an important mediator of bone remodeling. TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation. TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide(beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.
Synonyms: Transforming growth factor beta-1,TGF-beta-1,Latency-associated peptide,LAP,Tgfb1,
Full Gene Name: Transforming growth factor beta-1
Cellular Localisation: Secreted, extracellular space, extracellular matrix. - 基因ID
- 21803
- UniProt
- P04202
- 途径
- EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints
-
