TGFB1 ELISA 试剂盒
(23 references)-
- 抗原 See all TGFB1 ELISA试剂盒
- TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))
- 抗原表位
- AA 279-390
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Rat TGF beta 1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (EDTA), Urine
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: CHO
Immunogen sequence: A279-S390 - 交叉反应 （详细）
- There is cross-reactivity with TGFbeta2, TGFbeta3, TGFbeta5<1 % .
- 预测反应
- Bovine,Canine,Monkey,Rabbit
- 灵敏度
- <1pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: CHO
Immunogen sequence: A279-S390 - Featured
- Discover our best selling TGFB1 ELISA Kit
- Top Product
- Discover our top product TGFB1 ELISA Kit
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Transforming Growth Factor, beta 1 (TGFB1) ELISA Kit
TGFB1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 人 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma, Serum
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 人 AA 279-390 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum, Urine
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 小鼠 AA 279-390 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum, Urine
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 小鸡 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 犬 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 马 Colorimetric Competition ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Abundant in the bone matrix. .
- 板类型
- Pre-coated
- 实验流程
- rat TGF beta 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF beta 1 has been precoated onto 96-well plates. Standards(CHO, A279-S390) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF beta 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TGF beta 1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat TGF beta 1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum, plasma (EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat TGF beta 1 standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 122, Standard deviation: 4.15, CV(%): 3.4
- Sample 2: n=16, Mean(pg/ml): 253, Standard deviation: 11.4, CV(%): 4.5
- Sample 3: n=16, Mean(pg/ml): 506, Standard deviation: 25.81, CV(%): 5.1,
- Sample 1: n=24, Mean(pg/ml): 140, Standard deviation: 6.72, CV(%): 4.8
- Sample 2: n=24, Mean(pg/ml): 256, Standard deviation: 14.08, CV(%): 5.5
- Sample 3: n=24, Mean(pg/ml): 613, Standard deviation: 37.39, CV(%): 6.1
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Bacillus Calmette-Guerin alleviates airway inflammation and remodeling by preventing TGF-β1 induced epithelial-mesenchymal transition." in: Human vaccines & immunotherapeutics, Vol. 13, Issue 8, pp. 1758-1764, (2018) (PubMed).
: "Galectin-3 mediates pulmonary vascular remodeling in hypoxia-induced pulmonary arterial hypertension." in: Journal of the American Society of Hypertension : JASH, Vol. 11, Issue 10, pp. 673-683.e3, (2018) (PubMed).
: "Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes." in: BioMed research international, Vol. 2017, pp. 9538351, (2018) (PubMed).
: "Strychnos pseudoquina modulates the morphological reorganization of the scar tissue of second intention cutaneous wounds in rats." in: PLoS ONE, Vol. 13, Issue 4, pp. e0195786, (2018) (PubMed).
: "Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa β-related pathway in rats." in: Drug design, development and therapy, Vol. 12, pp. 1609-1619, (2018) (PubMed).
: "A New Oleanolic Acid Derivative against CCl₄-Induced Hepatic Fibrosis in Rats." in: International journal of molecular sciences, Vol. 18, Issue 3, (2017) (PubMed).
: "Nitric Oxide Synthase Activity Correlates with OGG1 in Ozone-Induced Lung Injury Animal Models." in: Frontiers in physiology, Vol. 8, pp. 249, (2017) (PubMed).
: "Efficacies of rosiglitazone and retinoin on bleomycin-induced pulmonary fibrosis in rats." in: Experimental and therapeutic medicine, Vol. 14, Issue 1, pp. 609-615, (2017) (PubMed).
: "Danggui buxue tang suppresses high glucose-induced proliferation and extracellular matrix accumulation of mesangial cells via inhibiting lncRNA PVT1." in: American journal of translational research, Vol. 9, Issue 8, pp. 3732-3740, (2017) (PubMed).
: "LSKL peptide alleviates subarachnoid fibrosis and hydrocephalus by inhibiting TSP1-mediated TGF-β1 signaling activity following subarachnoid hemorrhage in rats." in: Experimental and therapeutic medicine, Vol. 12, Issue 4, pp. 2537-2543, (2016) (PubMed).
: "3,4-dihydroxyphenylethanol suppresses irradiation-induced pulmonary fibrosis in adult rats." in: International journal of clinical and experimental pathology, Vol. 8, Issue 4, pp. 3441-50, (2015) (PubMed).
: "Protective effect of iridoid glycosides from Paederia scandens (LOUR.) MERRILL (Rubiaceae) on uric acid nephropathy rats induced by yeast and potassium oxonate." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 64, pp. 57-64, (2014) (PubMed).
: "Does vitamin D improve liver enzymes, oxidative stress, and inflammatory biomarkers in adults with non-alcoholic fatty liver disease? A randomized clinical trial." in: Endocrine, Vol. 47, Issue 1, pp. 70-80, (2014) (PubMed).
: "Over-production of nitric oxide by oxidative stress-induced activation of the TGF-β1/PI3K/Akt pathway in mesangial cells cultured in high glucose." in: Acta pharmacologica Sinica, Vol. 34, Issue 4, pp. 507-14, (2014) (PubMed).
: "Evening primrose oil ameliorates platelet aggregation and improves cardiac recovery in myocardial-infarct hypercholesterolemic rats." in: International journal of physiology, pathophysiology and pharmacology, Vol. 6, Issue 1, pp. 23-36, (2014) (PubMed).
: "The green tea extract epigallocatechin-3-gallate inhibits irradiation-induced pulmonary fibrosis in adult rats." in: International journal of molecular medicine, Vol. 34, Issue 1, pp. 92-102, (2014) (PubMed).
: "Resveratrol Ameliorates Diabetes-induced Renal Damage through Regulating the Expression of TGF-?1, Collagen IV and Th17/Treg-related Cytokines in Rats." in: The West Indian medical journal, Vol. 63, Issue 1, pp. 20-5, (2014) (PubMed).
: "NOX1 abet mesangial fibrogenesis via iNOS induction in diabetes." in: Molecular and cellular biochemistry, Vol. 382, Issue 1-2, pp. 185-91, (2013) (PubMed).
: "Correlation between apoptosis and TGF-β1 expression in the mucosal epithelium of rat small intestine in a cold stress state." in: Experimental and therapeutic medicine, Vol. 5, Issue 5, pp. 1456-1460, (2013) (PubMed).
: "The Ayurvedic Medicine Salacia oblonga Attenuates Diabetic Renal Fibrosis in Rats: Suppression of Angiotensin II/AT1 Signaling." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2011, pp. 807451, (2012) (PubMed).
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: "Bacillus Calmette-Guerin alleviates airway inflammation and remodeling by preventing TGF-β1 induced epithelial-mesenchymal transition." in: Human vaccines & immunotherapeutics, Vol. 13, Issue 8, pp. 1758-1764, (2018) (PubMed).
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- 抗原 See all TGFB1 ELISA试剂盒
- TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))
- 别名
- TGFB1 (TGFB1 产品)
- 别名
- CED ELISA Kit, DPD1 ELISA Kit, LAP ELISA Kit, TGFB ELISA Kit, TGFbeta ELISA Kit, TGF-beta ELISA Kit, TGF-BETA-1 ELISA Kit, TGF-beta5 ELISA Kit, ced ELISA Kit, dpd1 ELISA Kit, lap ELISA Kit, tgf-beta ELISA Kit, tgfb ELISA Kit, tgfb5 ELISA Kit, tgfbeta ELISA Kit, TGF-beta1 ELISA Kit, TGFbeta1 ELISA Kit, Tgfb ELISA Kit, Tgfb-1 ELISA Kit, ai39657 ELISA Kit, tgfb1 ELISA Kit, wu:fb13a07 ELISA Kit, xx:ai39657 ELISA Kit, TGFB1 ELISA Kit, csd ELISA Kit, cdb1 ELISA Kit, cdg2 ELISA Kit, csd1 ELISA Kit, csd2 ELISA Kit, csd3 ELISA Kit, ebmd ELISA Kit, lcd1 ELISA Kit, bigh3 ELISA Kit, cdgg1 ELISA Kit, betaig-h3 ELISA Kit, TGFB4 ELISA Kit, transforming growth factor beta 1 ELISA Kit, transforming growth factor beta-1 ELISA Kit, transforming growth factor beta 1 L homeolog ELISA Kit, transforming growth factor, beta 1 ELISA Kit, transforming growth factor, beta 1a ELISA Kit, transforming growth factor beta induced L homeolog ELISA Kit, TGFB1 ELISA Kit, Tgfb1 ELISA Kit, tgfb1.L ELISA Kit, tgfb1a ELISA Kit, tgfbi.L ELISA Kit
- 背景
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Protein Function: Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T- helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells (By similarity). .
Background: Transforming growth factor-beta1(TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects. TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation. TGFbeta1 is also an important mediator of bone remodeling. TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation. TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide(beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.
Synonyms: Transforming growth factor beta-1,TGF-beta-1,Latency-associated peptide,LAP,Tgfb1,
Full Gene Name: Transforming growth factor beta-1
Cellular Localisation: Secreted, extracellular space, extracellular matrix. - 基因ID
- 59086
- UniProt
- P17246
- 途径
- EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints
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