Selectin E/CD62e ELISA 试剂盒
(8 references)-
- 抗原 See all Selectin E/CD62e (SELE) ELISA试剂盒
- Selectin E/CD62e (SELE) (Selectin E (SELE))
- 抗原表位
- AA 22-556
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 125-8000 pg/mL
- 最低检测浓度
- 125 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human sE-Selectin
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: W22-P556 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <4pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: W22-P556 - Featured
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Selectin E (SELE) ELISA Kit
SELE 适用: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 人 Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Selectin E (SELE) ELISA KitSELE 适用: 兔 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 兔 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Selectin E (SELE) ELISA KitSELE 适用: 犬 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 小鼠 Soluble Colorimetric 78-5000 pg/mL Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 人 Soluble Colorimetric Sandwich ELISA 31 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
-
Sequence similarities: Belongs to the selectin/LECAM family.
- 板类型
- Pre-coated
- 实验流程
- human sE-Selectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for sE-Selectin has been precoated onto 96-well plates. Standards(NSO, W22-P556) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for sE-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human sE-Selectin amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human sE-Selectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human sE-Selectin standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(ng/ml): 1.7, Standard deviation: 0.10, CV(%): 5.9
- Sample 2: n=16, Mean(ng/ml): 3.1, Standard deviation: 0.16, CV(%): 5.2
- Sample 3: n=16, Mean(ng/ml): 4.5, Standard deviation: 0.31, CV(%): 6.9,
- Sample 1: n=24, Mean(ng/ml): 1.6, Standard deviation: 0.13, CV(%): 8.1
- Sample 2: n=24, Mean(ng/ml): 3.2, Standard deviation: 0.21, CV(%): 6.6
- Sample 3: n=24, Mean(ng/ml): 4.5, Standard deviation: 0.33, CV(%): 7.3
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
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: "Neurally mediated syncope: Is it really an endothelial dysfunction?" in: Anatolian journal of cardiology, Vol. 16, Issue 9, pp. 701-6, (2018) (PubMed).
: "Systemic and vascular inflammation in an in-vitro model of central obesity." in: PLoS ONE, Vol. 13, Issue 2, pp. e0192824, (2018) (PubMed).
: "Exercise-induced changes in stress hormones and cell adhesion molecules in obese men." in: Journal of inflammation research, Vol. 11, pp. 69-75, (2018) (PubMed).
: "Metoprolol Improves Endothelial Function in Patients with Cardiac Syndrome X." in: Iranian journal of pharmaceutical research : IJPR, Vol. 15, Issue 3, pp. 561-566, (2016) (PubMed).
: "Relationship of late arteriovenous fistula stenosis with soluble E-selectin and soluble EPCR in chronic hemodialysis patients with arteriovenous fistula." in: Clinical and experimental nephrology, Vol. 19, Issue 1, pp. 133-9, (2015) (PubMed).
: "Endothelial dysfunction state in migraine headache and neutrally mediated syncope in children and young adults." in: Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, Vol. 20, Issue 8, pp. 771-6, (2015) (PubMed).
: "Clinical investigation of the acute effects of pomegranate juice on blood pressure and endothelial function in hypertensive individuals." in: ARYA atherosclerosis, Vol. 9, Issue 6, pp. 326-31, (2014) (PubMed).
: "Effect of fresh orange juice intake on physiological characteristics in healthy volunteers." in: ISRN nutrition, Vol. 2014, pp. 405867, (2014) (PubMed).
-
: "Neurally mediated syncope: Is it really an endothelial dysfunction?" in: Anatolian journal of cardiology, Vol. 16, Issue 9, pp. 701-6, (2018) (PubMed).
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- 抗原 See all Selectin E/CD62e (SELE) ELISA试剂盒
- Selectin E/CD62e (SELE) (Selectin E (SELE))
- 别名
- SELE (SELE 产品)
- 别名
- SELE ELISA Kit, CD62E ELISA Kit, ELAM ELISA Kit, ELAM1 ELISA Kit, ESEL ELISA Kit, LECAM2 ELISA Kit, E-selectin ELISA Kit, Elam ELISA Kit, selectin E ELISA Kit, selectin, endothelial cell ELISA Kit, SELE ELISA Kit, Sele ELISA Kit
- 背景
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Protein Function: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. .
Background: E-selectin, also called endothelial-leukocyte adhesion molecule-1(ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells(HPCs) into bone marrow(BM). A structurally and functionally related group of genes, lymph node homing receptor(LHR), granule membrane protein 140(GMP-140), and endothelial leukocyte adhesion molecule 1(ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant human E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 535 amino acids with the molecular mass of 58.6KDa.
Synonyms: E-selectin,CD62 antigen-like family member E,Endothelial leukocyte adhesion molecule 1,ELAM-1,Leukocyte-endothelial cell adhesion molecule 2,LECAM2,CD62E,SELE,ELAM1,
Full Gene Name: E-selectin
Cellular Localisation: Cell membrane, Single-pass type I membrane protein. - 基因ID
- 6401
- UniProt
- P16581
- 途径
- Thromboxane A2 Receptor Signaling
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