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CCL3 ELISA 试剂盒

CCL3 适用: 人 AA 27-92 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
产品编号 ABIN411325
发货至: 中国
  • 抗原 See all CCL3 ELISA试剂盒
    CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
    抗原表位
    AA 27-92
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    15.6-1000 pg/mL
    最低检测浓度
    15.6 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MIP-1 alpha
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: E.coli
    Immunogen sequence: A27-A92
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <10pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: E.coli
    Immunogen sequence: A27-A92
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    板类型
    Pre-coated
    实验流程
    human MIP-1 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MIP-1 alpha has been precoated onto 96-well plates. Standards(E.coli, A27-A92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MIP-1 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MIP-1 alpha amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human MIP-1 alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MIP-1 alpha standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 107, Standard deviation: 4.39, CV(%): 4.1
    • Sample 2: n=16, Mean(pg/ml): 450, Standard deviation: 13.05, CV(%): 2.9
    • Sample 3: n=16, Mean(pg/ml): 732, Standard deviation: 25.62, CV(%): 3.5,
    • Sample 1: n=24, Mean(pg/ml): 95, Standard deviation: 6.84, CV(%): 7.2
    • Sample 2: n=24, Mean(pg/ml): 413, Standard deviation: 22.3, CV(%): 5.4
    • Sample 3: n=24, Mean(pg/ml): 682, Standard deviation: 34.78, CV(%): 5.1
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • 抗原 See all CCL3 ELISA试剂盒
    CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
    别名
    CCL3 (CCL3 产品)
    别名
    MIP-1alpha ELISA Kit, G0S19-1 ELISA Kit, LD78ALPHA ELISA Kit, MIP-1-alpha ELISA Kit, MIP1A ELISA Kit, SCYA3 ELISA Kit, MIP-1a ELISA Kit, Scya3 ELISA Kit, AI323804 ELISA Kit, LD78alpha ELISA Kit, MIP1-(a) ELISA Kit, MIP1-alpha ELISA Kit, Mip1a ELISA Kit, CCL3L1 ELISA Kit, CCL3L3 ELISA Kit, chemokine (C-C motif) ligand 3 ELISA Kit, C-C motif chemokine ligand 3 ELISA Kit, CCL3 ELISA Kit, Ccl3 ELISA Kit
    背景

    Background: Macrophage inflammatory protein-1 alpha(MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease. The standard product used in this kit is recombinant human MIP-1alpha, consisting of 66 amino acids with the molecular mass of 7.5 kDa.

    Synonyms: C-C motif chemokine ,CCL3 ,hCG_1749202 ,

    Full Gene Name: C-C motif chemokine

    Cellular Localisation: Secreted.
    基因ID
    6348
    UniProt
    A0N0R1
    途径
    Cellular Response to Molecule of Bacterial Origin, Autophagy
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