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IL-10 ELISA 试剂盒

Name: IL-10 ELISA 试剂盒
SKU: ABIN411308
Availability: OutOfStock

IL10 适用: 大鼠 AA 19-178 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum

ELISA

1 image

(66 references)

产品编号 ABIN411308

发货至: 中国

Datasheet as PDF

抗原 See all IL-10 (IL10) ELISA试剂盒

IL-10 (IL10) (Interleukin 10 (IL10))

抗原表位

AA 19-178
适用
28

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大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2-2000 pg/mL

最低检测浓度

31.2 pg/mL

应用范围

ELISA

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-10

品牌

PicoKine™

样品类型

Cell Culture Supernatant, Serum, Plasma (EDTA)

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: S19-N178

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<4pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: E.coli
Immunogen sequence: S19-N178

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应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

板类型

Pre-coated

实验流程

rat IL-10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-10 has been precoated onto 96-well plates. Standards(E.coli, S19-N178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-10 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat IL-10 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat IL-10 standard solution and each sample is measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 277, Standard deviation: 13.85, CV(%): 5
- Sample 2: n=16, Mean(pg/ml): 821, Standard deviation: 34.5, CV(%): 4.2
- Sample 3: n=16, Mean(pg/ml): 1344, Standard deviation: 52.42, CV(%): 3.9,
- Sample 1: n=24, Mean(pg/ml): 316, Standard deviation: 29.1, CV(%): 9.2
- Sample 2: n=24, Mean(pg/ml): 938, Standard deviation: 70.35, CV(%): 7.5
- Sample 3: n=24, Mean(pg/ml): 1620, Standard deviation: 144.2, CV(%): 8.9
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
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Liu, Xu, Yin, Wang, Wang, Xiao: "IL-10/STAT3 is reduced in childhood obesity with hypertriglyceridemia and is related to triglyceride level in diet-induced obese rats." in: BMC endocrine disorders, Vol. 18, Issue 1, pp. 39, (2018) (PubMed).

Ye, Shen, Huang, Zhang, Xiao: "Anti-fatigue activity of sea cucumber peptides prepared from Stichopus japonicus in an endurance swimming rat model." in: Journal of the science of food and agriculture, Vol. 97, Issue 13, pp. 4548-4556, (2017) (PubMed).

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Xu, Huang, Zhang, Li, Zheng, Yu, Chu, Lin, Chen: "Extracts of Bauhinia championii (Benth.) Benth. attenuate the inﬂammatory response in a rat model of collagen-induced arthritis." in: Molecular medicine reports, Vol. 13, Issue 5, pp. 4167-74, (2017) (PubMed).

Yu, Wang, He, Wang, Gao, Ren, Shi et al.: "Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling ..." in: Cell stress & chaperones, Vol. 21, Issue 4, pp. 697-705, (2017) (PubMed).

Mirmalek, Gholamrezaei Boushehrinejad, Yavari, Kardeh, Parsa, Salimi-Tabatabaee, Yadollah-Damavandi, Parsa, Shahverdi, Jangholi: "Antioxidant and Anti-Inflammatory Effects of Coenzyme Q10 on L-Arginine-Induced Acute Pancreatitis in Rat." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 5818479, (2017) (PubMed).

Zhao, Pan, Wang, Xu, Song: "Hyperbaric Oxygen Pretreatment Improves Cognition and Reduces Hippocampal Damage Via p38 Mitogen-Activated Protein Kinase in a Rat Model." in: Yonsei medical journal, Vol. 58, Issue 1, pp. 131-138, (2017) (PubMed).

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抗原 See all IL-10 (IL10) ELISA试剂盒

IL-10 (IL10) (Interleukin 10 (IL10))

别名

IL10 (IL10 产品)

别名

CSIF ELISA Kit, GVHDS ELISA Kit, IL-10 ELISA Kit, IL10A ELISA Kit, TGIF ELISA Kit, Il-10 ELISA Kit, IL10X ELISA Kit, interleukin 10 ELISA Kit, IL10 ELISA Kit, Il10 ELISA Kit

背景

Protein Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. .
Background: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.
Synonyms: Interleukin-10,IL-10,Cytokine synthesis inhibitory factor,CSIF,Il10,Il-10,
Full Gene Name: Interleukin-10
Cellular Localisation: Secreted.

基因ID

25325

UniProt

P29456

途径

Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints