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IL-10 ELISA 试剂盒

Name: IL-10 ELISA 试剂盒
SKU: ABIN411307
Availability: OutOfStock

IL10 适用: 小鼠 AA 19-178 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum

ELISA

1 image

(76 references)

产品编号 ABIN411307

发货至: 中国

Datasheet as PDF

抗原 See all IL-10 (IL10) ELISA试剂盒

IL-10 (IL10) (Interleukin 10 (IL10))

抗原表位

AA 19-178
适用
28

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6-1000 pg/mL

最低检测浓度

15.6 pg/mL

应用范围

ELISA

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-10

品牌

PicoKine™

样品类型

Cell Culture Supernatant, Serum

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: S19-S178

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<1pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: E.coli
Immunogen sequence: S19-S178

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应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

板类型

Pre-coated

实验流程

mouse IL-10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-10 has been precoated onto 96-well plates. Standards(E.coli, S19-S178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-10 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-10 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-10 standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 82, Standard deviation: 3.77, CV(%): 4.6
- Sample 2: n=16, Mean(pg/ml): 413, Standard deviation: 21.5, CV(%): 5.2
- Sample 3: n=16, Mean(pg/ml): 724, Standard deviation: 44.2, CV(%): 6.1,
- Sample 1: n=24, Mean(pg/ml): 113, Standard deviation: 7, CV(%): 6.2
- Sample 2: n=24, Mean(pg/ml): 367, Standard deviation: 24.96, CV(%): 6.8
- Sample 3: n=24, Mean(pg/ml): 628, Standard deviation: 46.5, CV(%): 7.4
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
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抗原 See all IL-10 (IL10) ELISA试剂盒

IL-10 (IL10) (Interleukin 10 (IL10))

别名

IL10 (IL10 产品)

别名

CSIF ELISA Kit, GVHDS ELISA Kit, IL-10 ELISA Kit, IL10A ELISA Kit, TGIF ELISA Kit, Il-10 ELISA Kit, IL10X ELISA Kit, interleukin 10 ELISA Kit, IL10 ELISA Kit, Il10 ELISA Kit

背景

Protein Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Background: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant mouse IL-10, consisting of 160 amino acid with the molecular mass of 18KDa.
Synonyms: Interleukin-10,IL-10,Cytokine synthesis inhibitory factor,CSIF,Il10,Il-10,
Full Gene Name: Interleukin-10
Cellular Localisation: Secreted.

基因ID

16153

UniProt

P18893

途径

Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints