IL-6 ELISA 试剂盒
(147 references)-
- 抗原 See all IL-6 (IL6) ELISA试剂盒
- IL-6 (IL6) (Interleukin 6 (IL6))
- 抗原表位
- AA 29-212
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 4.69-300 pg/mL
- 最低检测浓度
- 4.69 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-6
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: E.coli
Immunogen sequence: P29-M212 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <0.3pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: E.coli
Immunogen sequence: P29-M212 - Featured
- Discover our best selling IL6 ELISA Kit
- Top Product
- Discover our top product IL6 ELISA Kit
-
Interleukin 6 (IL6) ELISA Kit
IL6 适用: 人 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Interleukin 6 (IL6) ELISA KitIL6 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 6 (IL6) ELISA KitIL6 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 6 (IL6) ELISA KitIL6 适用: 大鼠 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 6 (IL6) ELISA KitIL6 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 6 (IL6) ELISA KitIL6 适用: 犬 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 6 (IL6) ELISA KitIL6 适用: 小鼠 AA 25-211 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 6 (IL6) ELISA KitIL6 适用: 大鼠 AA 25-211 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 6 (IL6) ELISA KitIL6 适用: 人 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 6 (IL6) ELISA KitIL6 适用: 兔 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- human IL-6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-6 has been precoated onto 96-well plates. Standards(E.coli, P29-M212) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-6 amount of sample captured in plate.
- 实验流程
-
Aliquot 0.1 mL per well of the 300pg/mL, 150pg/mL, 75pg/mL, 37.5pg/mL, 18.75pg/mL, 9.38pg/mL, 4.69pg/mL human IL-6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human IL-6 standard solution and each sample is measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 16.3, Standard deviation: 0.8, CV(%): 4.9
- Sample 2: n=16, Mean(pg/ml): 98, Standard deviation: 2.3, CV(%): 2.3
- Sample 3: n=16, Mean(pg/ml): 179, Standard deviation: 2.3, CV(%): 2.3,
- Sample 1: n=24, Mean(pg/ml): 18.2, Standard deviation: 1.0, CV(%): 5.5
- Sample 2: n=24, Mean(pg/ml): 99, Standard deviation: 3.6, CV(%): 3.6
- Sample 3: n=24, Mean(pg/ml): 185, Standard deviation: 5.7, CV(%): 3.1
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
-
: "LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling." in: Oncotarget, Vol. 7, Issue 39, pp. 63981-63994, (2018) (PubMed).
: "A wogonin-rich-fraction of Scutellaria baicalensis root extract exerts chondroprotective effects by suppressing IL-1β-induced activation of AP-1 in human OA chondrocytes." in: Scientific reports, Vol. 7, pp. 43789, (2018) (PubMed).
: "Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells." in: International journal of molecular medicine, Vol. 40, Issue 1, pp. 31-38, (2018) (PubMed).
: "Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA." in: Pediatric transplantation, Vol. 21, Issue 6, (2018) (PubMed).
: "Serum Heat Shock Protein Levels and the Relationship of Heat Shock Proteins with Various Parameters in Chronic Obstructive Pulmonary Disease Patients." in: Turkish thoracic journal, Vol. 17, Issue 4, pp. 153-159, (2018) (PubMed).
: "Cancer-associated Fibroblast-derived IL-6 Promotes Head and Neck Cancer Progression via the Osteopontin-NF-kappa B Signaling Pathway." in: Theranostics, Vol. 8, Issue 4, pp. 921-940, (2018) (PubMed).
: "Safety of Low-calcium Dialysate and its Effects on Coronary Artery Calcification in Patients Undergoing Maintenance Hemodialysis." in: Scientific reports, Vol. 8, Issue 1, pp. 5941, (2018) (PubMed).
: "Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis." in: Experimental and therapeutic medicine, Vol. 15, Issue 6, pp. 4671-4676, (2018) (PubMed).
: "Effect of fermented camel milk on glucose metabolism, insulin resistance, and inflammatory biomarkers of adolescents with metabolic syndrome: A double-blind, randomized, crossover trial." in: Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, Vol. 23, pp. 32, (2018) (PubMed).
: "The Relationship Between Serum Pentraxine 3 Levels and Hematological Markers in Patients With Rheumatoid Arthritis." in: Archives of rheumatology, Vol. 33, Issue 1, pp. 39-44, (2018) (PubMed).
: "Proteomics investigation of OSCC-specific salivary biomarkers in a Hungarian population highlights the importance of identification of population-tailored biomarkers." in: PLoS ONE, Vol. 12, Issue 5, pp. e0177282, (2017) (PubMed).
: "Sulfated Cyclocarya paliurus polysaccharides markedly attenuates inflammation and oxidative damage in lipopolysaccharide-treated macrophage cells and mice." in: Scientific reports, Vol. 7, pp. 40402, (2017) (PubMed).
: "Conditioned medium from relapsing-remitting multiple sclerosis patients reduces the expression and release of inflammatory cytokines induced by LPS-gingivalis in THP-1 and MO3.13 cell lines." in: Cytokine, Vol. 96, pp. 261-272, (2017) (PubMed).
: "ox-LDL increases microRNA-29a transcription through upregulating YY1 and STAT1 in macrophages." in: Cell biology international, Vol. 41, Issue 9, pp. 1001-1011, (2017) (PubMed).
: "C/EBP β Mediates Endoplasmic Reticulum Stress Regulated Inflammatory Response and Extracellular Matrix Degradation in LPS-Stimulated Human Periodontal Ligament Cells." in: International journal of molecular sciences, Vol. 17, Issue 3, pp. 385, (2017) (PubMed).
: "GEN-27, a Newly Synthetic Isoflavonoid, Inhibits the Proliferation of Colon Cancer Cells in Inflammation Microenvironment by Suppressing NF-κB Pathway." in: Mediators of inflammation, Vol. 2016, pp. 2853040, (2017) (PubMed).
: "Associations of Trauma Severity with Mean Platelet Volume and Levels of Systemic Inflammatory Markers (IL1β, IL6, TNFα, and CRP)." in: Mediators of inflammation, Vol. 2016, pp. 9894716, (2017) (PubMed).
: "NF-κB-Regulated miR-99a Modulates Endothelial Cell Inflammation." in: Mediators of inflammation, Vol. 2016, pp. 5308170, (2017) (PubMed).
: "miR-145 mediated the role of aspirin in resisting VSMCs proliferation and anti-inflammation through CD40." in: Journal of translational medicine, Vol. 14, Issue 1, pp. 211, (2017) (PubMed).
: "Pharmacological Inactivation of Src Family Kinases Inhibits LPS-Induced TNF-α Production in PBMC of Patients with Behçet's Disease." in: Mediators of inflammation, Vol. 2016, pp. 5414369, (2017) (PubMed).
-
: "LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling." in: Oncotarget, Vol. 7, Issue 39, pp. 63981-63994, (2018) (PubMed).
-
- 抗原 See all IL-6 (IL6) ELISA试剂盒
- IL-6 (IL6) (Interleukin 6 (IL6))
- 别名
- IL6 (IL6 产品)
- 别名
- BSF2 ELISA Kit, HGF ELISA Kit, HSF ELISA Kit, IFNB2 ELISA Kit, IL-6 ELISA Kit, Il-6 ELISA Kit, ILg6 ELISA Kit, Ifnb2 ELISA Kit, il6 ELISA Kit, CHIL-6 ELISA Kit, interleukin 6 ELISA Kit, interleukin-6 ELISA Kit, IL6 ELISA Kit, Il6 ELISA Kit, il-6 ELISA Kit, IL-6 ELISA Kit
- 背景
-
Background: The human interferon-beta 2 gene(IFNB2) product is identical to that for the B-cell stimulation factor-2(BSF-2), the hybridoma growth factor(HGF)("interleukin-6"), and the hepatocyte stimulating factor(HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury(acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6(IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3 kDa.
Synonyms: cDNA FLJ61423, highly similar to Interleukin-6 ,
Full Gene Name: interleukin 6
- 基因ID
- 3569
- UniProt
- B4DVM1
- 途径
- TLR signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
-
