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IL-4 ELISA 试剂盒

Name: IL-4 ELISA 试剂盒
SKU: ABIN411301
Availability: OutOfStock

IL4 适用: 大鼠 AA 25-147 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum

ELISA

1 image

(19 references)

产品编号 ABIN411301

发货至: 中国

Datasheet as PDF

抗原 See all IL-4 (IL4) ELISA试剂盒

IL-4 (IL4) (Interleukin 4 (IL4))

抗原表位

AA 25-147
适用
20

17

14

6

6

5

5

4

4

4

4

3

2

2

2

2

2

2

1

1
大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6-1000 pg/mL

最低检测浓度

15.6 pg/mL

应用范围

ELISA

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-4

品牌

PicoKine™

样品类型

Cell Culture Supernatant, Serum

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: C25-S147

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<1pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: E.coli
Immunogen sequence: C25-S147

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应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

板类型

Pre-coated

实验流程

rat IL-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-4 has been precoated onto 96-well plates. Standards(E.coli, C25-S147) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-4 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat IL-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat IL-4 standard solution and each sample is measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 54.7, Standard deviation: 3.23, CV(%): 5.9
- Sample 2: n=16, Mean(pg/ml): 428, Standard deviation: 21.83, CV(%): 5.1
- Sample 3: n=16, Mean(pg/ml): 738, Standard deviation: 31.73, CV(%): 4.3,
- Sample 1: n=24, Mean(pg/ml): 53.8, Standard deviation: 4.79, CV(%): 8.9
- Sample 2: n=24, Mean(pg/ml): 387, Standard deviation: 25.93, CV(%): 6.7
- Sample 3: n=24, Mean(pg/ml): 729, Standard deviation: 45.2, CV(%): 6.2
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
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抗原 See all IL-4 (IL4) ELISA试剂盒

IL-4 (IL4) (Interleukin 4 (IL4))

别名

IL4 (IL4 产品)

别名

IL4 ELISA Kit, IL-4 ELISA Kit, BCGF-1 ELISA Kit, BCGF1 ELISA Kit, BSF-1 ELISA Kit, BSF1 ELISA Kit, Il-4 ELISA Kit, Il4e12 ELISA Kit, IL2 ELISA Kit, interleukin 4 ELISA Kit, IL4 ELISA Kit, Il4 ELISA Kit

背景

Protein Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
Background: Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. Rat IL-4 is a 13.5KDa glycoprotein, consisting of 147 amino acids in precursor form and 123 amino acids in mature form.
Synonyms: Interleukin-4,IL-4,B-cell IGG differentiation factor,B-cell growth factor 1,B-cell stimulatory factor 1,BSF-1,Lymphocyte stimulatory factor 1,Il4,Il-4,
Full Gene Name: Interleukin-4
Cellular Localisation: Secreted.

基因ID

287287

UniProt

P20096

途径

JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation