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IL-2 ELISA 试剂盒

Name: IL-2 ELISA 试剂盒
SKU: ABIN411296
Availability: OutOfStock

IL2 适用: 小鼠 AA 21-169 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

ELISA

1 image

(26 references)

产品编号 ABIN411296

发货至: 中国

Datasheet as PDF

抗原 See all IL-2 (IL2) ELISA试剂盒

IL-2 (IL2) (Interleukin 2 (IL2))

抗原表位

AA 21-169
适用
22

20

13

7

5

4

4

4

3

3

2

2

2

2

2

2

1

1

1
小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6-1000 pg/mL

最低检测浓度

15.6 pg/mL

应用范围

ELISA

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-2

品牌

PicoKine™

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli,A21-Q169

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<1pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: E.coli
Immunogen sequence: A21-Q169

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应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

板类型

Pre-coated

实验流程

mouse IL-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-2 has been precoated onto 96-well plates. Standards (E.coli,A21-Q169) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-2 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-2 standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 81, Standard deviation: 4.536, CV(%): 5.6
- Sample 2: n=16, Mean(pg/ml): 215, Standard deviation: 11.61, CV(%): 5.4
- Sample 3: n=16, Mean(pg/ml): 590, Standard deviation: 25.37, CV(%): 4.3,
- Sample 1: n=24, Mean(pg/ml): 86, Standard deviation: 5.246, CV(%): 6.1
- Sample 2: n=24, Mean(pg/ml): 193, Standard deviation: 13.32, CV(%): 6.9
- Sample 3: n=24, Mean(pg/ml): 575, Standard deviation: 29.9, CV(%): 5.2
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
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抗原 See all IL-2 (IL2) ELISA试剂盒

IL-2 (IL2) (Interleukin 2 (IL2))

别名

IL2 (IL2 产品)

别名

IL2 ELISA Kit, IL-2 ELISA Kit, il2 ELISA Kit, TCGF ELISA Kit, lymphokine ELISA Kit, Il-2 ELISA Kit, POIL2 ELISA Kit, interleukin 2 ELISA Kit, interleukin-15 ELISA Kit, IL2 ELISA Kit, il2 ELISA Kit, LOC100303688 ELISA Kit, Il2 ELISA Kit

背景

Protein Function: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells.
Background: Interleukin-2(IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2(IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2(IL2) is assigned to chromosome 4. Mouse IL-2 contains 149 amino acids in mature form with the molecular mass of 17.2KDa.
Synonyms: Interleukin-2,IL-2,T-cell growth factor,TCGF,Il2,Il-2,
Full Gene Name: Interleukin-2
Cellular Localisation: Secreted.

基因ID

16183

UniProt

P04351

途径

JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation