IL1A ELISA 试剂盒
(13 references)-
- 抗原 See all IL1A ELISA试剂盒
- IL1A (Interleukin 1 alpha (IL1A))
- 抗原表位
- AA 115-270
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 4.69-300 pg/mL
- 最低检测浓度
- 4.69 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-1 alpha
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- Expression system for standard: E.coli,S115-S270
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <0.5pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: S115-S270 - Featured
- Discover our best selling IL1A ELISA Kit
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- Discover our top product IL1A ELISA Kit
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Interleukin 1 alpha (IL1A) ELISA Kit
IL1A 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitHigh Sensitivity IL1A 适用: 山羊 Colorimetric Sandwich ELISA 0.781 pg/mL - 50 pg/mL Plasma, Serum
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 人 Colorimetric Sandwich ELISA 3.91 pg/mL - 250 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 犬 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 小鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 小鸡 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: Hamster Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- rat IL-1 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1 alpha has been precoated onto 96-well plates. Standards (E.coli,S115-S270) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-1 alpha amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 300pg/mL, 150pg/mL, 75pg/mL, 37.5pg/mL, 18.8pg/mL, 9.4pg/mL, 4.69pg/mL rat IL-1 alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat IL-1 alpha standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 45, Standard deviation: 1.89, CV(%): 4.2
- Sample 2: n=16, Mean(pg/ml): 137, Standard deviation: 6.58, CV(%): 4.8
- Sample 3: n=16, Mean(pg/ml): 211, Standard deviation: 10.76, CV(%): 5.1,
- Sample 1: n=24, Mean(pg/ml): 49, Standard deviation: 2.646, CV(%): 5.4
- Sample 2: n=24, Mean(pg/ml): 145, Standard deviation: 8.99, CV(%): 6.2
- Sample 3: n=24, Mean(pg/ml): 230, Standard deviation: 15.41, CV(%): 6.7
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Anti-inflammatory effects of Lefty-1 in renal tubulointerstitial inflammation via regulation of the NF-κB pathway." in: International journal of molecular medicine, Vol. 41, Issue 3, pp. 1293-1304, (2018) (PubMed).
: "Cytokine Attenuation and Free Radical Scavenging Activity of a New Flavanone7,4'-Dihydroxy-3″,3″-Dimethyl -(5,6-Pyrano-2″-One)- 8- (3‴,3‴-Dimethyl Allyl)- Isolated from Mallotus philippensis: ..." in: PLoS ONE, Vol. 11, Issue 12, pp. e0167294, (2017) (PubMed).
: "Anti-inflammatory effect of Fumaria parviflora leaves based on TNF-α, IL-1, IL-6 and antioxidant potential." in: Avicenna journal of phytomedicine, Vol. 7, Issue 1, pp. 37-45, (2017) (PubMed).
: "Inhibitory Effects of Angelica Polysaccharide on Activation of Mast Cells." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 6063475, (2016) (PubMed).
: "Anti-inflammatory activity of roots of Cichorium intybus due to its inhibitory effect on various cytokines and antioxidant activity." in: Ancient science of life, Vol. 34, Issue 1, pp. 44-9, (2015) (PubMed).
: "The association of oxidative stress and pro-inflammatory cytokines in diabetic patients with hyperglycemic crisis." in: Journal of diabetes and its complications, Vol. 28, Issue 5, pp. 662-6, (2014) (PubMed).
: "Anti-fatigue and vasoprotective effects of quercetin-3-O-gentiobiose on oxidative stress and vascular endothelial dysfunction induced by endurance swimming in rats." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 68, pp. 290-6, (2014) (PubMed).
: "Synergistic interaction between choline and aspirin against acute inflammation induced by carrageenan and lipopolysaccharide." in: International immunopharmacology, Vol. 20, Issue 1, pp. 229-37, (2014) (PubMed).
: "Brain microglia were activated in sporadic CJD but almost unchanged in fatal familial insomnia and G114V genetic CJD." in: Virology journal, Vol. 10, pp. 216, (2014) (PubMed).
: "Baicalin attenuates brain edema in a rat model of intracerebral hemorrhage." in: Inflammation, Vol. 37, Issue 1, pp. 107-15, (2014) (PubMed).
: "Functional blockage of EMMPRIN ameliorates atherosclerosis in apolipoprotein E-deficient mice." in: International journal of cardiology, Vol. 168, Issue 4, pp. 3248-53, (2013) (PubMed).
: "HSV-1 miR-H6 inhibits HSV-1 replication and IL-6 expression in human corneal epithelial cells in vitro." in: Clinical & developmental immunology, Vol. 2012, pp. 192791, (2012) (PubMed).
: "[Present state and improvement of the methods of preventing ORL diseases in industrial workers]." in: Vestnik otorinolaringologii, Issue 2, pp. 3-8, (1985) (PubMed).
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: "Anti-inflammatory effects of Lefty-1 in renal tubulointerstitial inflammation via regulation of the NF-κB pathway." in: International journal of molecular medicine, Vol. 41, Issue 3, pp. 1293-1304, (2018) (PubMed).
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- 抗原 See all IL1A ELISA试剂盒
- IL1A (Interleukin 1 alpha (IL1A))
- 别名
- IL1A (IL1A 产品)
- 别名
- IL-1A ELISA Kit, IL1 ELISA Kit, IL1-ALPHA ELISA Kit, IL1F1 ELISA Kit, IL-6 ELISA Kit, Il-1a ELISA Kit, IL-1 alpha ELISA Kit, IL-1alpha ELISA Kit, L1A ELISA Kit, interleukin 1 alpha ELISA Kit, interleukin 6 ELISA Kit, IL1A ELISA Kit, IL6 ELISA Kit, Il1a ELISA Kit
- 背景
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Protein Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Background: Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant rat IL-1alpha with the molecular mass of 18 kDa.
Synonyms: Interleukin-1 alpha,IL-1 alpha,Il1a,
Full Gene Name: Interleukin-1 alpha
Cellular Localisation: Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. - 基因ID
- 24493
- UniProt
- P16598
- 途径
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
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