ICAM1 ELISA 试剂盒
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- 抗原 See all ICAM1 ELISA试剂盒
- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
- 抗原表位
- AA 28-480
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ICAM-1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: Q28-E480 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: Q28-E480 - Featured
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Intercellular Adhesion Molecule 1 (ICAM1) ELISA Kit
ICAM1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 犬 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 兔 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 小鼠 AA 28-485 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 大鼠 AA 28-493 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 猴 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitSmall Sample ICAM1 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
-
Sequence similarities: Belongs to the immunoglobulin superfamily. ICAM family.
- 板类型
- Pre-coated
- 实验流程
- human ICAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ICAM-1 has been precoated onto 96-well plates. Standards(NSO, Q28-E480) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ICAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ICAM-1 amount of sample captured in plate.
- 实验流程
-
Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human ICAM-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ICAM-1 standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(ng/ml): 1.41, Standard deviation: 0.048, CV(%): 3.4
- Sample 2: n=16, Mean(ng/ml): 2.3, Standard deviation: 0.106, CV(%): 4.6
- Sample 3: n=16, Mean(ng/ml): 5.2, Standard deviation: 0.265, CV(%): 5.1,
- Sample 1: n=24, Mean(ng/ml): 1.33, Standard deviation: 0.047, CV(%): 6.4
- Sample 2: n=24, Mean(ng/ml): 2.27, Standard deviation: 0.252, CV(%): 5.6
- Sample 3: n=24, Mean(ng/ml): 5, Standard deviation: 0.314, CV(%): 4.1
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
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: "Neurally mediated syncope: Is it really an endothelial dysfunction?" in: Anatolian journal of cardiology, Vol. 16, Issue 9, pp. 701-6, (2018) (PubMed).
: "Effects of Therapy with Maraviroc on the Carotid Intima Media Thickness in HIV-1/HCV Co-infected Patients." in: In vivo (Athens, Greece), Vol. 31, Issue 1, pp. 125-131, (2017) (PubMed).
: "FGF-23/Vitamin D Axis in Type 1 Diabetes: The Potential Role of Mineral Metabolism in Arterial Stiffness." in: PLoS ONE, Vol. 10, Issue 10, pp. e0140222, (2016) (PubMed).
: "Comparison of the effects of Crataegus oxyacantha extract, aerobic exercise and their combination on the serum levels of ICAM-1 and E-Selectin in patients with stable angina pectoris." in: Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences, Vol. 23, pp. 54, (2016) (PubMed).
: "Evaluation of the effect of Vaccinium arctostaphylos L. fruit extract on serum inflammatory biomarkers in adult hyperlipidemic patients: a randomized double-blind placebo-controlled clinical trial." in: Research in pharmaceutical sciences, Vol. 11, Issue 4, pp. 343-8, (2016) (PubMed).
: "Is there interaction between gut microbial profile and cardiovascular risk in chronic kidney disease patients?" in: Future microbiology, Vol. 10, Issue 4, pp. 517-26, (2015) (PubMed).
: "Comparison of protective effects of safflor injection and extract of Ginkgo biloba on lung ischemia/reperfusion injury in rabbits." in: Chinese journal of integrative medicine, Vol. 21, Issue 3, pp. 229-33, (2015) (PubMed).
: "Endothelial dysfunction state in migraine headache and neutrally mediated syncope in children and young adults." in: Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, Vol. 20, Issue 8, pp. 771-6, (2015) (PubMed).
: "Upregulation of ICAM-1 in diabetic rats after transient forebrain ischemia and reperfusion injury." in: Journal of inflammation (London, England), Vol. 11, Issue 1, pp. 35, (2014) (PubMed).
: "EETs alleviate ox-LDL-induced inflammation by inhibiting LOX-1 receptor expression in rat pulmonary arterial endothelial cells." in: European journal of pharmacology, Vol. 727, pp. 43-51, (2014) (PubMed).
: "Inhibitory effect of Malvidin on TNF-?-induced inflammatory response in endothelial cells." in: European journal of pharmacology, Vol. 723, pp. 67-72, (2014) (PubMed).
: "Clinical investigation of the acute effects of pomegranate juice on blood pressure and endothelial function in hypertensive individuals." in: ARYA atherosclerosis, Vol. 9, Issue 6, pp. 326-31, (2014) (PubMed).
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: "Investigation of the lipid-modifying and antiinflammatory effects of Cornus mas L. supplementation on dyslipidemic children and adolescents." in: Pediatric cardiology, Vol. 34, Issue 7, pp. 1729-35, (2013) (PubMed).
: "Apelin-APJ induces ICAM-1, VCAM-1 and MCP-1 expression via NF-?B/JNK signal pathway in human umbilical vein endothelial cells." in: Amino acids, Vol. 43, Issue 5, pp. 2125-36, (2012) (PubMed).
: "Phytoestrogen calycosin-7-O-?-D-glucopyranoside ameliorates advanced glycation end products-induced HUVEC damage." in: Journal of cellular biochemistry, Vol. 112, Issue 10, pp. 2953-65, (2011) (PubMed).
: "Crocetin inhibits leukocyte adherence to vascular endothelial cells induced by AGEs." in: Journal of ethnopharmacology, Vol. 107, Issue 1, pp. 25-31, (2006) (PubMed).
: "Time courses of vascular endothelial growth factor and intercellular adhesion molecule-1 expressions in aortas of atherosclerotic rats." in: Life sciences, Vol. 77, Issue 20, pp. 2529-39, (2005) (PubMed).
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: "Neurally mediated syncope: Is it really an endothelial dysfunction?" in: Anatolian journal of cardiology, Vol. 16, Issue 9, pp. 701-6, (2018) (PubMed).
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- 抗原 See all ICAM1 ELISA试剂盒
- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
- 别名
- ICAM1 (ICAM1 产品)
- 别名
- BB2 ELISA Kit, CD54 ELISA Kit, P3.58 ELISA Kit, Icam-1 ELISA Kit, Ly-47 ELISA Kit, MALA-2 ELISA Kit, ICAM ELISA Kit, ICAM-1 ELISA Kit, ICAM1 ELISA Kit, intercellular adhesion molecule 1 ELISA Kit, intercellular adhesion molecule-1 ELISA Kit, ICAM1 ELISA Kit, Icam1 ELISA Kit, ICAM-1 ELISA Kit
- 物质类
- Viral Protein
- 背景
-
Protein Function: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans- endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. .
Background: Intercellular adhesion molecule-1(ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated(LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1(ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen(LFA-1) or macrophage-1 antigen(Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1
Synonyms: Intercellular adhesion molecule 1,ICAM-1,Major group rhinovirus receptor,CD54,ICAM1,
Full Gene Name: Intercellular adhesion molecule 1
Cellular Localisation: Membrane, Single-pass type I membrane protein. - 基因ID
- 3383
- UniProt
- P05362
- 途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
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