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CX3CL1 ELISA 试剂盒

CX3CL1 适用: 人 AA 25-339 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
产品编号 ABIN411273
发货至: 中国
  • 抗原 See all CX3CL1 ELISA试剂盒
    CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))
    抗原表位
    AA 25-339
    适用
    • 9
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    156-10000 pg/mL
    最低检测浓度
    156 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Fractalkine/CX3CL1
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine, Milk
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: NSO
    Immunogen sequence: Q25-R339
    交叉反应 (详细)
    There is detectable cross-reactivity with Eotaxin, MCP-1, MCP-2, 6Ckine, MARC< 0.2 % .
    灵敏度
    <50pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: NSO
    Immunogen sequence: Q25-R339
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    板类型
    Pre-coated
    实验流程
    human Fractalkine ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Fractalkine has been precoated onto 96-well plates. Standards(NSO, Q25-R339) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Fractalkine is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fractalkine amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Fractalkine standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine or human milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Fractalkine standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(ng/ml): 1.5, Standard deviation: 0.063, CV(%): 4.2
    • Sample 2: n=16, Mean(ng/ml): 3.8, Standard deviation: 0.122, CV(%): 3.2
    • Sample 3: n=16, Mean(ng/ml): 6.6, Standard deviation: 0.31, CV(%): 4.7,
    • Sample 1: n=24, Mean(ng/ml): 1.3, Standard deviation: 0.111, CV(%): 8.5
    • Sample 2: n=24, Mean(ng/ml): 3.7, Standard deviation: 0.237, CV(%): 6.4
    • Sample 3: n=24, Mean(ng/ml): 6.5, Standard deviation: 0.403, CV(%): 6.2
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • 抗原 See all CX3CL1 ELISA试剂盒
    CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))
    别名
    CX3CL1 (CX3CL1 产品)
    别名
    ABCD3 ELISA Kit, CX3CL1 ELISA Kit, DKFZp459K117 ELISA Kit, Cx3c ELISA Kit, Scyd1 ELISA Kit, ABCD-3 ELISA Kit, C3Xkine ELISA Kit, CXC3 ELISA Kit, CXC3C ELISA Kit, NTN ELISA Kit, NTT ELISA Kit, SCYD1 ELISA Kit, fractalkine ELISA Kit, neurotactin ELISA Kit, AB030188 ELISA Kit, AI848747 ELISA Kit, CX3C ELISA Kit, Cxc3 ELISA Kit, D8Bwg0439e ELISA Kit, ATP binding cassette subfamily D member 3 ELISA Kit, C-X3-C motif chemokine ligand 1 ELISA Kit, chemokine (C-X3-C motif) ligand 1 ELISA Kit, ABCD3 ELISA Kit, CX3CL1 ELISA Kit, Cx3cl1 ELISA Kit, Abcd3 ELISA Kit
    背景

    Background: Chemokine(C-X3-C motif) ligand 1(CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine(in humans) and neurotactin(in mice). CX3CL1 is produced as a long protein(with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble(90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22. It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.

    Synonyms: Chemokine (C-X3-C motif) ligand 1 ,cDNA, FLJ94424, Homo sapiens chemokine (C-X3-C motif) ligand 1 (CX3CL1), mRNA ,CX3CL1 ,hCG_15105 ,

    Full Gene Name: chemokine (C-X3-C motif) ligand 1

    基因ID
    6376
    UniProt
    A0N0N7
    途径
    Synaptic Membrane
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