ELANE ELISA 试剂盒
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- 抗原 See all ELANE ELISA试剂盒
- ELANE (Elastase, Neutrophil Expressed (ELANE))
- 抗原表位
- AA 27-260
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Elastase
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: S27-R260 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
- Immunogen sequence: S27-R260
- Featured
- Discover our best selling ELANE ELISA Kit
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- Discover our top product ELANE ELISA Kit
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Elastase, Neutrophil Expressed (ELANE) ELISA Kit
ELANE 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Elastase, Neutrophil Expressed (ELANE) ELISA KitELANE 适用: 人 Colorimetric Sandwich ELISA 0.19 ng/mL - 12 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Elastase, Neutrophil Expressed (ELANE) ELISA KitELANE 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Elastase, Neutrophil Expressed (ELANE) ELISA KitELANE 适用: 犬 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Elastase, Neutrophil Expressed (ELANE) ELISA KitELANE 适用: 大鼠 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Elastase, Neutrophil Expressed (ELANE) ELISA KitELANE 适用: 大鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Elastase, Neutrophil Expressed (ELANE) ELISA KitELANE 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Elastase, Neutrophil Expressed (ELANE) ELISA KitELANE 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
Elastase, Neutrophil Expressed (ELANE) ELISA KitELANE 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Elastase, Neutrophil Expressed (ELANE) ELISA KitELANE 适用: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- mouse Elastase ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Elastase has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: S27-R260) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Elastase is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Elastase amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse Elastase standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Elastase standard solution and each sample be measured in duplicate.
- 限制
- 仅限研究用
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- 缓冲液
- heparin or EDTA
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all ELANE ELISA试剂盒
- ELANE (Elastase, Neutrophil Expressed (ELANE))
- 别名
- ELANE (ELANE 产品)
- 别名
- ELA2 ELISA Kit, GE ELISA Kit, HLE ELISA Kit, HNE ELISA Kit, NE ELISA Kit, PMN-E ELISA Kit, SCN1 ELISA Kit, Ela2 ELISA Kit, F430011M15Rik ELISA Kit, ela2 ELISA Kit, ELANE ELISA Kit, elastase, neutrophil expressed ELISA Kit, proteinase 3 ELISA Kit, ELANE ELISA Kit, Elane ELISA Kit, prtn3 ELISA Kit
- 背景
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Protein Function: Medullasin modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (By similarity). .
Background: Neutrophil elastase is a serine protease of neutrophil and monocyte granules. Its key physiologic role is in innate host defense, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.
Synonyms: Neutrophil elastase,3.4.21.37,Elastase-2,Leukocyte elastase,Elane,Ela2,
Full Gene Name: Neutrophil elastase
- 基因ID
- 50701
- UniProt
- Q3UP87
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