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CCL28 ELISA 试剂盒

CCL28 适用: 人 AA 23-127 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
产品编号 ABIN3044695
发货至: 中国
  • 抗原 See all CCL28 ELISA试剂盒
    CCL28 (Chemokine (C-C Motif) Ligand 28 (CCL28))
    抗原表位
    AA 23-127
    适用
    • 6
    • 3
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    31.2-2000 pg/mL
    最低检测浓度
    31.2 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL28
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: E.coli
    Immunogen sequence: I23-Y127
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <10pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Immunogen sequence: I23-Y127
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Tissue Specificity: Preferentially expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. Also found in prostate, spleen, thyroid, psoriasis skin and in lower levels in peripheral blood leukocytes, small intestine, Peyer patches, stomach and normal skin.

    板类型
    Pre-coated
    实验流程
    human CCL28 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL28 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: I23-Y127) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL28 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL28 amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CCL28 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CCL28 standard solution and each sample be measured in duplicate.

    限制
    仅限研究用
  • 缓冲液
    heparin or EDTA
    注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • 抗原 See all CCL28 ELISA试剂盒
    CCL28 (Chemokine (C-C Motif) Ligand 28 (CCL28))
    别名
    CCL28 (CCL28 产品)
    别名
    Scya28 ELISA Kit, CCL28 ELISA Kit, ccl28 ELISA Kit, nrpb ELISA Kit, enc-1 ELISA Kit, pig10 ELISA Kit, Encr-3 ELISA Kit, klhl35 ELISA Kit, Xencr-3 ELISA Kit, tp53i10 ELISA Kit, LOC100303767 ELISA Kit, CCK1 ELISA Kit, MEC ELISA Kit, SCYA28 ELISA Kit, C-C motif chemokine ligand 28 ELISA Kit, ectodermal-neural cortex 1 (with BTB domain) ELISA Kit, C-C motif chemokine 28 ELISA Kit, chemokine (C-C motif) ligand 28 ELISA Kit, Ccl28 ELISA Kit, CCL28 ELISA Kit, ccl28 ELISA Kit, enc1 ELISA Kit, LOC100303767 ELISA Kit
    背景

    Protein Function: Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner.

    Background: CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T (Treg) cells through induction of expression of the chemokine CCL28, which in turn promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.

    Synonyms: C-C motif chemokine 28,Mucosae-associated epithelial chemokine,MEC,Protein CCK1,Small-inducible cytokine A28,CCL28,SCYA28,

    Full Gene Name: C-C motif chemokine 28

    Cellular Localisation: Secreted.
    基因ID
    56477
    UniProt
    Q9NRJ3
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