Pseudomonas Exotoxin A ELISA 试剂盒
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- 抗原 See all Pseudomonas Exotoxin A (PEA) products
- Pseudomonas Exotoxin A (PEA)
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 原理
- Human Pseudomonas Exotoxin A ELISA Kit is an ELISA kit against Human Pseudomonas Exotoxin A.
- 样品类型
- Serum, Plasma
- Analytical Method
- Quantitative
- 灵敏度
- < 0.094 ng/mL
- 组件
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1. One 96-well plate pre-coated with anti-Human PEA antibody
2. Lyophilized Human PEA standards: 2 tubes
3. Sample / Standard diluent buffer: 20ml
4. Biotin conjugated anti-Human PEA antibody (Concentrated): 120 μl. Dilution: 1:100
5. Antibody diluent buffer: 10 ml
6. HRP Streptavidin Conjugate (SABC) (Concentrated): 120 μl. Dilution: 1:100
7. SABC diluent buffer: 10 ml
8. TMB substrate: 10 ml
9. Stop solution: 10 ml
10. Wash buffer (25X): 30 ml - 试剂未包括
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1. 37°C incubator
2. Microplate reader (wavelength: 450nm)
3. Pipettes and disposable pipette tips
4. Automated plate washer (optional)
5. ELISA shaker
6. 1.5 ml tubes
7. Plate cover
8. Absorbent filter papers
9. Plastic or glass container with volume greater than 1L
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Pseudomonas Exotoxin A (PEA) ELISA Kit
PEA 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Saliva, Serum, Tissue Homogenate
Pseudomonas Exotoxin A (PEA) ELISA KitPEA 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Pseudomonas Exotoxin A (PEA) ELISA KitPEA 适用: 人 Colorimetric Sandwich ELISA 0.156-10 ng/mL Saliva, Serum, Tissue Homogenate
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- 应用备注
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Stability: The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
Recommended dilutions: Optimal dilutions/concentrations should be determined by the end user.
Standard Form: Lyophilized
- 板类型
- Pre-coated
- 实验流程
- This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PEA antibody is pre-coated onto 96-well plates. Biotin conjugated anti-PEA monoclonal antibody is used as a detection antibody. The standards, test samples and biotin conjugated detection antibody are added to the wells and washed with wash buffer. HRP Streptavidin is added and unbound conjugates are washed away with wash buffer. TMB substrate is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue colour product that changes into yellow after adding stop solution. The density of yellow is proportional to the PEA amount of sample captured in plate. The O.D. absorbance is measured spectrophotometrically at 450nm in a microplate reader, and then the concentration of PEA can be calculated.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
- 储存方法
- Upon receipt, store the kit according to the storage instruction in the kit's manual.
- 有效期
- 6 months
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- 抗原 See all Pseudomonas Exotoxin A (PEA) products
- Pseudomonas Exotoxin A (PEA)
- 别名
- Pseudomonas Exotoxin A (PEA 产品)
- 背景
- The Pseudomonas exotoxin (or exotoxin A) is an exotoxin produced by Pseudomonas aeruginosa. It inhibits elongation factor-2. It does so by ADPribosylation of EF2. This then causes the elongation of polypeptides to cease. The mechanism of the toxin is similar to that of Diphtheria toxin. It has been investigated as a treatment for hepatitis B and cancer.
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