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Endomorphin 1 FLISA Kit

EM1 适用: 人 Fluorometric Competition ELISA 29.7-940 pg/mL
产品编号 ABIN2871394
发货至: 中国
  • 抗原 See all Endomorphin 1 (EM1) products
    Endomorphin 1 (EM1)
    适用
    检测方法
    Fluorometric
    实验类型
    Competition ELISA
    检测范围
    29.7-940 pg/mL
    最低检测浓度
    29.7 pg/mL
    应用范围
    ELISA
    Analytical Method
    Quantitative
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
    限制
    仅限研究用
  • 储存条件
    4 °C
  • 抗原 See all Endomorphin 1 (EM1) products
    Endomorphin 1 (EM1)
    别名
    Endomorphin-1 (EM1 产品)
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