Urocortin FLISA Kit
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- 抗原 See all Urocortin (UCN) FLISA Kits
- Urocortin (UCN)
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适用
- 大鼠
- 检测方法
- Fluorometric
- 实验类型
- Competition ELISA
- 应用范围
- ELISA
- Analytical Method
- Quantitative
- Top Product
- Discover our top product UCN ELISA Kit
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Urocortin (UCN) ELISA Kit
UCN 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Urocortin (UCN) ELISA KitUCN 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Urocortin (UCN) ELISA KitUCN 适用: 小鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Urocortin (UCN) ELISA KitUCN 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
- The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all Urocortin (UCN) FLISA Kits
- Urocortin (UCN)
- 别名
- Urocortin (UCN 产品)
- 别名
- UCN FLISA Kit, si:ch211-243j20.4 FLISA Kit, ucn FLISA Kit, wu:fq17a01 FLISA Kit, UI FLISA Kit, UROC FLISA Kit, Mpv17 FLISA Kit, Ucn1 FLISA Kit, urocortin FLISA Kit, urotensin 1 FLISA Kit, UCN FLISA Kit, uts1 FLISA Kit, Ucn FLISA Kit
- 途径
- Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of Cell Size, Feeding Behaviour
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