Spexin FLISA Kit
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- 抗原 See all Spexin (SPX) products
- Spexin (SPX)
- 适用
- 人, Cow, 小鼠
- 检测方法
- Fluorometric
- 实验类型
- Competition ELISA
- 应用范围
- ELISA
- Analytical Method
- Quantitative
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- Discover our best selling SPX ELISA Kit
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Spexin (SPX) ELISA Kit
SPX 适用: 人 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Spexin (SPX) ELISA KitSPX 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
- The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all Spexin (SPX) products
- Spexin (SPX)
- 别名
- Spexin/NPQ (SPX 产品)
- 别名
- Npq FLISA Kit, Spx FLISA Kit, spexin hormone FLISA Kit, SPX FLISA Kit, Spx FLISA Kit
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