Ghrelin CLIA Kit
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- 抗原 See all Ghrelin (GHRL) CLIA Kits
- Ghrelin (GHRL)
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Competition ELISA
- 应用范围
- ELISA
- Analytical Method
- Quantitative
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- Discover our top product GHRL ELISA Kit
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
- The immunoplate in this kit is pre-coated with secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate solution. The luminescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all Ghrelin (GHRL) CLIA Kits
- Ghrelin (GHRL)
- 别名
- Ghrelin (GHRL 产品)
- 别名
- 2210006E23Rik CLIA Kit, Ghr CLIA Kit, MTLRP CLIA Kit, MTLRPAP CLIA Kit, m46 CLIA Kit, Gh1-1 CLIA Kit, GRHL CLIA Kit, MTLRP-AP CLIA Kit, GHR CLIA Kit, ghrelin CLIA Kit, ghrelin and obestatin prepropeptide CLIA Kit, ghrelin/obestatin prepropeptide CLIA Kit, Ghrl CLIA Kit, GHRL CLIA Kit, ghrl CLIA Kit
- 途径
- Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Peptide Hormone Metabolism, Negative Regulation of Hormone Secretion, Synaptic Membrane, Feeding Behaviour
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