beta Endorphin FLISA Kit
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- 抗原 See all beta Endorphin (beta-EP) FLISA Kits
- beta Endorphin (beta-EP) (Endorphin beta (beta-EP))
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适用
- 小鼠, Cow, 绵羊, Camel
- 检测方法
- Fluorometric
- 实验类型
- Competition ELISA
- 应用范围
- ELISA
- Analytical Method
- Quantitative
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- Discover our best selling beta-EP ELISA Kit
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
- The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all beta Endorphin (beta-EP) FLISA Kits
- beta Endorphin (beta-EP) (Endorphin beta (beta-EP))
- 别名
- beta-Endorphin (beta-EP 产品)
- 别名
- ACTH FLISA Kit, BE FLISA Kit, Beta-LPH FLISA Kit, Clip FLISA Kit, Gamma-LPH FLISA Kit, Npp FLISA Kit, Pomc-1 FLISA Kit, Pomc1 FLISA Kit, alpha-MSH FLISA Kit, alphaMSH FLISA Kit, beta-MSH FLISA Kit, gamma-MSH FLISA Kit, CLIP FLISA Kit, LPH FLISA Kit, MSH FLISA Kit, NPP FLISA Kit, POC FLISA Kit, pro-opiomelanocortin-alpha FLISA Kit, proopiomelanocortin FLISA Kit, Pomc FLISA Kit, POMC FLISA Kit
- 途径
- Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity
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