T-Bet ELISA 试剂盒
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- 抗原 See all T-Bet ELISA试剂盒
- T-Bet
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- DNA-Binding ELISA
- 应用范围
- ELISA
- 原理
- DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.
- 品牌
- TransAM®
- 样品类型
- Cell Extracts, Tissue Samples
- Analytical Method
- Quantitative
- 特异性
- TransAM T-bet Kits are tested for sensitivity in detecting T-bet activation.
- 产品特性
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Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. - 组件
- One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.
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T-Bet ELISA Kit
适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
T-Bet ELISA Kit适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
T-Bet ELISA Kit适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Nuclear extracts prepared from Cos-7 cells that are either transfected with T-bet or not and stimulated with PMA/ionomycin. Nuclear extracts are diluted to 0.156 μg/well and assayed using the TransAM T-bet Kit. The ratio of the signals from the stimulated over unstimulated cells must be above 4. Lot No. 29408001 was developed for 3 minutes. It gave a ratio of 120 (Figure 1). This ratio may vary depending on the level of T-bet activation in a given cell type. TransAM T-bet Kits are also tested for specificity in detecting T-bet activation. TransAM T-bet assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated T-bet consensus binding site (Figure 2). At 40X excess, the wild-type oligonucleotide prevents T-bet binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has no effect on T-bet binding.
- 实验时间
- 5 h
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
- Store the cell extract at -80°C. Other kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the oligonucleotides, DTT, poly [d(I-C)] and Protease Inhibitor Cocktail that should be kept at -20°C. This product is guaranteed for 6 months from date of receipt.
- 有效期
- 6 months
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- 抗原 See all T-Bet ELISA试剂盒
- T-Bet
- Abstract
- T-Bet 产品
- 别名
- T-PET ELISA Kit, T-bet ELISA Kit, TBET ELISA Kit, TBLYM ELISA Kit, TBT1 ELISA Kit, Tbet ELISA Kit, Tblym ELISA Kit, T-box 21 ELISA Kit, TBX21 ELISA Kit, Tbx21 ELISA Kit
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