PPARG ELISA 试剂盒
-
- 抗原 See all PPARG ELISA试剂盒
- PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- DNA-Binding ELISA
- 应用范围
- ELISA
- 原理
- DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.
- 品牌
- TransAM®
- 样品类型
- Cell Extracts, Tissue Samples
- Analytical Method
- Quantitative
- 特异性
- TransAM® PPARγ Kits are tested for sensitivity in detecting PPARγ in nuclear extracts from COS-7 cells that are either untransfected or transfected with recombinant PPARγ.
- 产品特性
-
Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. - 组件
- One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.
- Featured
- Discover our best selling PPARG ELISA Kit
- Top Product
- Discover our top product PPARG ELISA Kit
-
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA Kit
PPARG 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG 适用: 兔 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG 适用: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
These extracts are diluted down to 0.625 μg/well and assayed using the TransAM PPARγ Kit. The ratio of the signals from the transfected cells over the untransfected cells must be above 3. Lot No. 24114005 was developed for 15 minutes. It gave a ratio of 7.6 (Figure 1). The basal level of PPARγ expression, and this ratio, may vary depending on the cell type tested and the stimulation used. TransAM PPARγ Kits are also tested for specificity. TransAM PPARγ assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated PPARγ consensus binding site (Figure 2). At 40 pmol, the wild-type oligonucleotide prevents PPARγ binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has little effect on PPARγ binding.
- 实验时间
- 5 h
- 板类型
- Pre-coated
- 限制
- 仅限研究用
-
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
- Store the cell extract at -80°C. All other components should be stored at -20°C prior to first use. Then, store them at 4°C, except for the primary and secondary antibodies, oligonucleotides, DTT and Protease Inhibitor Cocktail, which should be kept at -20°C, and the cell extract, which should be stored at -80°C. This product is guaranteed for 6 months from date of receipt.
- 有效期
- 6 months
-
- 抗原 See all PPARG ELISA试剂盒
- PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))
- 别名
- Pparg (PPARG 产品)
- 别名
- PPAR gamma ELISA Kit, PPARg2 ELISA Kit, PPARG ELISA Kit, Nr1c3 ELISA Kit, PPAR-gamma ELISA Kit, PPAR-gamma2 ELISA Kit, PPARgamma ELISA Kit, PPARgamma2 ELISA Kit, NR1C3 ELISA Kit, CIMT1 ELISA Kit, GLM1 ELISA Kit, PPARG1 ELISA Kit, PPARG2 ELISA Kit, xPPAR-gamma ELISA Kit, PPAR-GAMMA ELISA Kit, PPARGAMMA ELISA Kit, peroxisome proliferator-activated receptor gamma ELISA Kit, peroxisome proliferator activated receptor gamma ELISA Kit, peroxisome proliferator activated receptor gamma L homeolog ELISA Kit, PPARG ELISA Kit, Pparg ELISA Kit, pparg.L ELISA Kit
- 途径
- MAPK Pathway, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
-
