p53 ELISA 试剂盒
-
- 抗原 See all p53 (TP53) ELISA试剂盒
- p53 (TP53) (Tumor Protein P53 (TP53))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- DNA-Binding ELISA
- 应用范围
- ELISA
- 原理
- DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.
- 品牌
- TransAM®
- 样品类型
- Cell Extracts, Tissue Samples
- Analytical Method
- Quantitative
- 特异性
- TransAM p53 Kits are tested for sensitivity in detecting p53 in nuclear extracts from MCF-7 cells that are either untreated or treated for 3 hours with 200 μM H2O2.
- 产品特性
-
Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. - 组件
- One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.
- Featured
- Discover our best selling TP53 ELISA Kit
- Top Product
- Discover our top product TP53 ELISA Kit
-
Tumor Protein P53 (TP53) ELISA Kit
TP53 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 人 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 人 Colorimetric Sandwich ELISA 9.38 pg/mL - 600 pg/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Serum, Tissue Homogenate, Urine
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 人 acetylated Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 人 AA 1-393 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Lysate
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
These extracts are diluted down to 0.625 μg/well and assayed using the TransAM p53 Kit. The ratio of the signals from the treated over the untreated cells must be above 4. Lot 08614019 was developed for 3 minutes. It gave a ratio of 4.3 (Figure 1). The basal level of p53 expression, and this ratio, may vary depending on the cell type tested and the treatment used. TransAM p53 Kits are also tested for specificity in detecting p53 activation. TransAM p53 assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated p53 consensus binding site (Figure 2). At 20X excess, the wild-type oligonucleotide prevents p53 binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has little effect on p53 binding.
- 实验时间
- 5 h
- 板类型
- Pre-coated
- 限制
- 仅限研究用
-
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
- Except for the cell extract that must be kept at -80°C, the kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the oligonucleotides, DTT, poly[d(I-C)], primary antibody and Protease Inhibitor Cocktail that should be kept at -20°C, and the cell extract at -80°C. This product is guaranteed for 6 months from date of receipt.
- 有效期
- 6 months
-
- 抗原 See all p53 (TP53) ELISA试剂盒
- p53 (TP53) (Tumor Protein P53 (TP53))
- 别名
- p53 (TP53 产品)
- 别名
- BCC7 ELISA Kit, LFS1 ELISA Kit, P53 ELISA Kit, TRP53 ELISA Kit, p53 ELISA Kit, Trp53 ELISA Kit, brp53 ELISA Kit, drp53 ELISA Kit, etID22686.5 ELISA Kit, fb40d06 ELISA Kit, wu:fb40d06 ELISA Kit, zgc:111919 ELISA Kit, Tp53 ELISA Kit, bbl ELISA Kit, bfy ELISA Kit, bhy ELISA Kit, p44 ELISA Kit, tp53 ELISA Kit, Xp53 ELISA Kit, TP53 ELISA Kit, tumor protein p53 ELISA Kit, cellular tumor antigen p53 ELISA Kit, transformation related protein 53 ELISA Kit, tumor protein p53 L homeolog ELISA Kit, TP53 ELISA Kit, CpipJ_CPIJ002758 ELISA Kit, Tp53 ELISA Kit, tp53 ELISA Kit, Trp53 ELISA Kit, tp53.L ELISA Kit
- 途径
- p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
-
