c-MYC ELISA 试剂盒
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- 抗原 See all c-MYC (MYC) ELISA试剂盒
- c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- DNA-Binding ELISA
- 应用范围
- ELISA
- 原理
- DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.
- 品牌
- TransAM®
- 样品类型
- Cell Extracts, Tissue Samples
- Analytical Method
- Quantitative
- 特异性
- TransAM c-Myc Kits are tested for sensitivity in detecting c-Myc activation.
- 产品特性
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Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. - 组件
- One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.
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Myc Proto-Oncogene protein (MYC) ELISA Kit
MYC 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Myc Proto-Oncogene protein (MYC) ELISA KitMYC 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Myc Proto-Oncogene protein (MYC) ELISA KitMYC 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Myc Proto-Oncogene protein (MYC) ELISA KitMYC 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
Myc Proto-Oncogene protein (MYC) ELISA KitMYC 适用: 人 Colorimetric Sandwich ELISA 0.312-20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Nuclear extracts prepared from unstimulated Jurkat (1 day growth) and U-937 cells are diluted to 0.625 μg/well and assayed using the TransAM c-Myc Kit. The ratio of the signals from the Jurkat cells over the U-937 cells must be above 4. Lot No. 06012008 was developed for 2.5 minutes. It gave a ratio of 403.5 (Figure 1). The endogenous level of c-Myc expression, and this ratio may vary depending on the cell type tested and the treatment used. TransAM c-Myc Kits are also tested for specificity in detecting c-Myc activity. TransAM c-Myc assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated c-Myc consensus binding site (Figure 2). At 20X excess, the wild-type oligonucleotide prevents c-Myc binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has no effect on c-Myc binding.
- 实验时间
- 5 h
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
- Store the cell extract at -80°C. Other kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the oligonucleotides, primary antibody, DTT and Protease Inhibitor Cocktail that should be kept at -20°C. This product is guaranteed for 6 months from date of receipt.
- 有效期
- 6 months
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- 抗原 See all c-MYC (MYC) ELISA试剂盒
- c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))
- 别名
- C-Myc (MYC 产品)
- 别名
- MRTL ELISA Kit, MYCC ELISA Kit, bHLHe39 ELISA Kit, c-Myc ELISA Kit, CMYC ELISA Kit, c-myc ELISA Kit, C-MYC ELISA Kit, LOC100136746 ELISA Kit, ARC ELISA Kit, B430311C09Rik ELISA Kit, MYC ELISA Kit, NOP ELISA Kit, Nop30 ELISA Kit, RNCMYC ELISA Kit, mMyc ELISA Kit, AU016757 ELISA Kit, Myc2 ELISA Kit, Niard ELISA Kit, Nird ELISA Kit, cmyc ELISA Kit, zc-myc ELISA Kit, c-myc II ELISA Kit, myc-B ELISA Kit, myc2 ELISA Kit, myc1 ELISA Kit, CMyc ELISA Kit, zgc:55680 ELISA Kit, CG10798 ELISA Kit, D-Myc ELISA Kit, DM ELISA Kit, DMYc ELISA Kit, Dm ELISA Kit, Dmel\CG10798 ELISA Kit, Dmyc ELISA Kit, EG:BACN5I9.1 ELISA Kit, anon-WO03040301.171 ELISA Kit, bHLHe57 ELISA Kit, c-MYC ELISA Kit, d-myc ELISA Kit, dMYC ELISA Kit, dMyc ELISA Kit, dMyc1 ELISA Kit, da ELISA Kit, dm ELISA Kit, dm/dMyc ELISA Kit, dm/myc ELISA Kit, dmyc ELISA Kit, dmyc1 ELISA Kit, l(1)G0139 ELISA Kit, l(1)G0354 ELISA Kit, l(1)G0359 ELISA Kit, myc ELISA Kit, MYC proto-oncogene, bHLH transcription factor ELISA Kit, v-myc myelocytomatosis viral oncogene homolog ELISA Kit, nucleolar protein 3 (apoptosis repressor with CARD domain) ELISA Kit, myelocytomatosis oncogene ELISA Kit, MYC proto-oncogene, bHLH transcription factor a ELISA Kit, MYC proto-oncogene, bHLH transcription factor L homeolog ELISA Kit, MYC proto-oncogene, bHLH transcription factor S homeolog ELISA Kit, v-myc myelocytomatosis viral oncogene homolog (avian) ELISA Kit, MYC proto-oncogene, bHLH transcription factor b ELISA Kit, CG10798 gene product from transcript CG10798-RB ELISA Kit, MYC ELISA Kit, LOC100136746 ELISA Kit, Nol3 ELISA Kit, Myc ELISA Kit, myca ELISA Kit, myc.L ELISA Kit, myc.S ELISA Kit, mycb ELISA Kit
- 途径
- p53 Pathway, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect
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