ATF2 ELISA 试剂盒
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- 抗原 See all ATF2 ELISA试剂盒
- ATF2 (Activating Transcription Factor 2 (ATF2))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- DNA-Binding ELISA
- 应用范围
- ELISA
- 原理
- DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.
- 品牌
- TransAM®
- 样品类型
- Cell Extracts, Tissue Samples
- Analytical Method
- Quantitative
- 特异性
- TransAM ATF-2 Kits are tested for sensitivity in detecting ATF-2 activation.
- 产品特性
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Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. - 组件
- One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.
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- Discover our best selling ATF2 ELISA Kit
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- Discover our top product ATF2 ELISA Kit
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Activating Transcription Factor 2 (ATF2) ELISA Kit
ATF2 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Activating Transcription Factor 2 (ATF2) ELISA KitATF2 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Activating Transcription Factor 2 (ATF2) ELISA KitATF2 适用: 人, 大鼠, 小鼠 pSer386 Colorimetric Sandwich ELISA Cell Culture Lysate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Nuclear extracts prepared from untreated and treated HeLa (Anisomycin) cells are diluted to 0.625 μg/well and assayed using the TransAM ATF-2 Kit. The ratio of the signals from the treated cells over the untreated must be above 4. Lot No. 25209003 was developed for 7 minutes. It gave a ratio of 4.1 (Figure 1). The endogenous level of ATF-2 expression, and this ratio may vary depending on the cell type tested and the treatment used. TransAM ATF-2 Kits are also tested for specificity in detecting ATF-2 activity. TransAM ATF-2 assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated ATF-2 consensus binding site (Figure 2). At 20X excess, the wild-type oligonucleotide prevents ATF-2 binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has no effect on ATF-2 binding.
- 实验时间
- 5 h
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
- Store the cell extract at -80°C. Other kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the oligonucleotides, DTT and Protease Inhibitor Cocktail that should be kept at -20°C. This product is guaranteed for 6 months from date of receipt.
- 有效期
- 6 months
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- 抗原 See all ATF2 ELISA试剂盒
- ATF2 (Activating Transcription Factor 2 (ATF2))
- 别名
- Atf-2 (ATF2 产品)
- 别名
- CRE-BP1 ELISA Kit, CREB2 ELISA Kit, HB16 ELISA Kit, TREB7 ELISA Kit, CG12850 ELISA Kit, CG3749 ELISA Kit, dATF-2 ELISA Kit, DmelCG30420 ELISA Kit, CG30420 ELISA Kit, CHN1 ELISA Kit, Atf-2 ELISA Kit, CRE-BP ELISA Kit, Creb2 ELISA Kit, D130078H02Rik ELISA Kit, D18875 ELISA Kit, Tg(Gzma-Klra1)7Wum ELISA Kit, mXBP ELISA Kit, atf2-A ELISA Kit, atf2l ELISA Kit, activating transcription factor 2 ELISA Kit, Activating transcription factor-2 ELISA Kit, activating transcription factor 2 S homeolog ELISA Kit, ATF2 ELISA Kit, Atf-2 ELISA Kit, Atf2 ELISA Kit, atf2.S ELISA Kit, atf2 ELISA Kit
- 途径
- MAPK Pathway, RTK signaling, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades
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