Chemerin ELISA 试剂盒
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- 抗原 See all Chemerin (RARRES2) ELISA试剂盒
- Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))
- 抗原表位
- AA 20-162
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.78-50 ng/mL
- 最低检测浓度
- 0.78 ng/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Chemerin/RARRES2
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: T20-K162 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <20pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: T20-K162 - Featured
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- Discover our top product RARRES2 ELISA Kit
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Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA Kit
RARRES2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 大鼠 Colorimetric Sandwich ELISA 0.9 ng/mL - 60 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 人 AA 17-163 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Expressed in the differentiated adipocytes (at protein level). Abundantly expressed in the liver, adipose tissue including visceral, epididymal, and brown adipose tissue. .
- 板类型
- Pre-coated
- 实验流程
- mouse Chemerin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Chemerin has been precoated onto 96-well plates. Standards(E.coli, T20-K162) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Chemerin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Chemerin amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse Chemerin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Chemerin standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 3.9, Standard deviation: 0.199, CV(%): 5.1
- Sample 2: n=16, Mean(ng/ml): 16, Standard deviation: 0.752, CV(%): 4.7
- Sample 3: n=16, Mean(ng/ml): 27, Standard deviation: 0.999, CV(%): 3.7,
- Sample 1: n=24, Mean(ng/ml): 4, Standard deviation: 0.34, CV(%): 8.5
- Sample 2: n=24, Mean(ng/ml): 17.8, Standard deviation: 1.23, CV(%): 6.9
- Sample 3: n=24, Mean(ng/ml): 30, Standard deviation: 1.62, CV(%): 5.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all Chemerin (RARRES2) ELISA试剂盒
- Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))
- 别名
- RARRES2 (RARRES2 产品)
- 别名
- HP10433 ELISA Kit, TIG2 ELISA Kit, 0610007L05Rik ELISA Kit, AI303516 ELISA Kit, retinoic acid receptor responder 2 ELISA Kit, retinoic acid receptor responder (tazarotene induced) 2 ELISA Kit, RARRES2 ELISA Kit, Rarres2 ELISA Kit
- 背景
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Protein Function: Adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1). Its other ligands include G protein-coupled receptor 1 (GPR1) and chemokine receptor-like 2 (CCRL2). Positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. Also acts as a proinflammatory adipokine, causing an increase in secretion of proinflammatory and prodiabetic adipokines, which further impair adipose tissue metabolic function and have negative systemic effects including impaired insulin sensitivity, altered glucose and lipid metabolism, and a decrease in vascular function in other tissues. Can have both pro- and anti-inflammatory properties depending on the modality of enzymatic cleavage by different classes of proteases. Acts as a chemotactic factor for leukocyte populations expressing CMKLR1, particularly immature plasmacytoid dendritic cells, but also immature myeloid DCs, macrophages and natural killer cells. Exerts an anti-inflammatory role by preventing TNF/TNFA-induced VCAM1 expression and monocytes adhesion in vascular endothelial cells. The effect is mediated via inhibiting activation of NF-kappa-B and CRK/p38 through stimulation of AKT1/NOS3 signaling and nitric oxide production. Exhibits an antimicrobial function in the skin. .
Background: Chemerin, also known as RARRES2 or TIG2, is a protein that is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1(also known as ChemR23). It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What’s more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.
Synonyms: Retinoic acid receptor responder protein 2,Chemerin,Rarres2,
Full Gene Name: Retinoic acid receptor responder protein 2
Cellular Localisation: Secreted. - 基因ID
- 71660
- UniProt
- Q9DD06
- 途径
- Brown Fat Cell Differentiation
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