IGF2R ELISA 试剂盒
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- 抗原 See all IGF2R ELISA试剂盒
- IGF2R (Insulin-Like Growth Factor 2 Receptor (IGF2R))
- 抗原表位
- AA 1510-2108
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10.000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGF2R
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: S1510-F2108 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: S1510-F2108 - Featured
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Insulin-Like Growth Factor 2 Receptor (IGF2R) ELISA Kit
IGF2R 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 2 Receptor (IGF2R) ELISA KitIGF2R 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Insulin-Like Growth Factor 2 Receptor (IGF2R) ELISA KitIGF2R 适用: 人 Colorimetric Sandwich ELISA 0.27-200 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the MRL1/IGF2R family.
- 板类型
- Pre-coated
- 实验流程
- human IGF2R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGF2R has been precoated onto 96-well plates. Standards(NSO, S1510-F2108) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF2R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGF2R amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IGF2R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGF2R standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.8, Standard deviation: 0.063, CV(%): 3.5
- Sample 2: n=16, Mean(ng/ml): 4.2, Standard deviation: 0.172, CV(%): 4.1
- Sample 3: n=16, Mean(ng/ml): 6.7, Standard deviation: 0.389, CV(%): 5.8,
- Sample 1: n=24, Mean(ng/ml): 1.5, Standard deviation: 0.065, CV(%): 4.3
- Sample 2: n=24, Mean(ng/ml): 3.9, Standard deviation: 0.222, CV(%): 5.7
- Sample 3: n=24, Mean(ng/ml): 6.4, Standard deviation: 0.397, CV(%): 6.2
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all IGF2R ELISA试剂盒
- IGF2R (Insulin-Like Growth Factor 2 Receptor (IGF2R))
- 别名
- IGF2R (IGF2R 产品)
- 别名
- CD222 ELISA Kit, CIMPR ELISA Kit, M6P-R ELISA Kit, MPR1 ELISA Kit, MPRI ELISA Kit, AI661837 ELISA Kit, CI-MPR ELISA Kit, M6P/IGF2R ELISA Kit, Mpr300 ELISA Kit, mpri ELISA Kit, cd222 ELISA Kit, cimpr ELISA Kit, m6p-r ELISA Kit, insulin like growth factor 2 receptor ELISA Kit, insulin-like growth factor 2 receptor ELISA Kit, IGF2R ELISA Kit, Igf2r ELISA Kit, igf2r ELISA Kit
- 背景
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Protein Function: Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6- phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. .
Background: Insulin-like growth factor 2 receptor, also called IGF2R or I-MPR is a protein that in humans is encoded by the IGF2R gene. This gene is mapped to 6q25.3. This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the maternal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the maternal allele.
Synonyms: Cation-independent mannose-6-phosphate receptor,CI Man-6-P receptor,CI-MPR,M6PR,300 kDa mannose 6-phosphate receptor,MPR 300,Insulin-like growth factor 2 receptor,Insulin-like growth factor II receptor,IGF-II receptor,M6P/IGF2 receptor,M6P/IGF2R,CD222,IGF2R,MPRI,
Full Gene Name: Cation-independent mannose-6-phosphate receptor
Cellular Localisation: Lysosome membrane, Single-pass type I membrane protein . Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface. - 基因ID
- 3482
- UniProt
- P11717
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