Kallikrein 12 ELISA 试剂盒
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- 抗原 See all Kallikrein 12 (KLK12) ELISA试剂盒
- Kallikrein 12 (KLK12) (Kallikrein-Related Peptidase 12 (KLK12))
- 抗原表位
- AA 18-248
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10.000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human KLK12
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
- NSO, A18-N248
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: A18-N248 - Featured
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Kallikrein-Related Peptidase 12 (KLK12) ELISA Kit
KLK12 适用: 人 Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- human KLK12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK12 has been precoated onto 96-well plates. Standards (NSO, A18-N248) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK12 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human KLK12 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 1409, Standard deviation: 73.26, CV(%): 5.2
- Sample 2: n=16, Mean(pg/ml): 3882, Standard deviation: 213.51, CV(%): 5.5
- Sample 3: n=16, Mean(pg/ml): 6756, Standard deviation: 405.36, CV(%): 6.0,
- Sample 1: n=24, Mean(pg/ml): 1523, Standard deviation: 89.85, CV(%): 5.9
- Sample 2: n=24, Mean(pg/ml): 4129, Standard deviation: 251.86, CV(%): 6.1
- Sample 3: n=24, Mean(pg/ml): 6526, Standard deviation: 443.76, CV(%): 6.8
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all Kallikrein 12 (KLK12) ELISA试剂盒
- Kallikrein 12 (KLK12) (Kallikrein-Related Peptidase 12 (KLK12))
- 别名
- KLK12 (KLK12 产品)
- 别名
- KLK12 ELISA Kit, 2310008B01Rik ELISA Kit, AI324852 ELISA Kit, KLK-L5 ELISA Kit, KLKL5 ELISA Kit, kallikrein-12 ELISA Kit, kallikrein related-peptidase 12 ELISA Kit, kallikrein related peptidase 12 ELISA Kit, LOC611766 ELISA Kit, Klk12 ELISA Kit, KLK12 ELISA Kit
- 背景
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Background: Kallikrein-12 is a protein that in humans is encoded by the KLK12 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. KLK12 is expressed primarily in salivary gland, stomach, uterus, trachea, prostate, thymus, lung, colon, brain, breast, and thyroid gland and at lower levels in testis, pancreas, small intestine, and spinal cord. The expression of KLK12 is downregulated at the mRNA level in breast cancer tissues and is upregulated by steroid hormones in breast and prostate cancer cell lines.
Synonyms: Kallikrein-12,3.4.21.-,Kallikrein-like protein 5,KLK-L5,KLK12,KLKL5,UNQ669/PRO1303,
Full Gene Name: Kallikrein-12
Cellular Localisation: Secreted. - 基因ID
- 43849
- UniProt
- Q9UKR0
- 途径
- Complement System
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