Kallikrein 11 ELISA 试剂盒
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- 抗原 See all Kallikrein 11 (KLK11) ELISA试剂盒
- Kallikrein 11 (KLK11)
- 抗原表位
- AA 19-250
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10.000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human KLK11
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
- NSO, E19-N250
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: E19-N250 - Featured
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Kallikrein 11 (KLK11) ELISA Kit
KLK11 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level). .
- 板类型
- Pre-coated
- 实验流程
- human KLK11 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK11 has been precoated onto 96-well plates. Standards (NSO,E19-N250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK11 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK11 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK11 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human KLK11 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 1399, Standard deviation: 68.55, CV(%): 4.9
- Sample 2: n=16, Mean(pg/ml): 3520, Standard deviation: 204.16, CV(%): 5.8
- Sample 3: n=16, Mean(pg/ml): 6635, Standard deviation: 364.92, CV(%): 5.5,
- Sample 1: n=24, Mean(pg/ml): 1575, Standard deviation: 92.92, CV(%): 5.9
- Sample 2: n=24, Mean(pg/ml): 4236, Standard deviation: 279.57, CV(%): 6.6
- Sample 3: n=24, Mean(pg/ml): 6751, Standard deviation: 398.30, CV(%): 5.9
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all Kallikrein 11 (KLK11) ELISA试剂盒
- Kallikrein 11 (KLK11)
- 别名
- KLK11 (KLK11 产品)
- 别名
- KLK11 ELISA Kit, 2310015I08Rik ELISA Kit, Prss20 ELISA Kit, TLSP ELISA Kit, PRSS20 ELISA Kit, kallikrein related peptidase 11 ELISA Kit, kallikrein related-peptidase 11 ELISA Kit, kallikrein-related peptidase 11 ELISA Kit, KLK11 ELISA Kit, Klk11 ELISA Kit
- 背景
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Protein Function: Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. .
Background: Kallikrein-11 is a protein that in humans is encoded by the KLK11 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in two transcript variants encoding two different isoforms which are differentially expressed.
Synonyms: Kallikrein-11,hK11,3.4.21.-,Hippostasin,Serine protease 20,Trypsin-like protease,Kallikrein-11 inactive chain 1,Kallikrein-11 inactive chain 2,KLK11,PRSS20, TLSP,UNQ649/PRO1279,
Full Gene Name: Kallikrein-11
Cellular Localisation: Isoform 1: Secreted. - 基因ID
- 11012
- UniProt
- Q9UBX7
- 途径
- Complement System
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