KNG1 ELISA 试剂盒
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- 抗原 See all KNG1 ELISA试剂盒
- KNG1 (Kininogen 1 (KNG1))
- 抗原表位
- AA 19-644
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.78-50 ng/mL
- 最低检测浓度
- 0.78 ng/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Kininogen-1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: Q19-S644 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <20pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: Q19-S644 - Featured
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- Discover our top product KNG1 ELISA Kit
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Kininogen 1 (KNG1) ELISA Kit
KNG1 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 大鼠 Colorimetric Competition ELISA 1.25 ng/mL - 80 ng/mL Cell Culture Supernatant, Plasma, Serum
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 人 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 大鼠 Colorimetric Competition ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 人 Colorimetric Competition ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
Kininogen 1 (KNG1) ELISA KitKNG1 适用: Pig Colorimetric Competition ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 小鼠 Colorimetric Competition ELISA 1.23 pg/mL - 100 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 小鼠 Colorimetric Sandwich ELISA 0.25 ng/mL - 16 ng/mL Plasma
Kininogen 1 (KNG1) ELISA KitHigh Sensitivity KNG1 适用: 人 Colorimetric Competition ELISA 1.23 pg/mL - 100 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Contains 3 cystatin kininogen-type domains.
Tissue Specificity: Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors. .
- 板类型
- Pre-coated
- 实验流程
- human Kininogen-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kininogen-1 has been precoated onto 96-well plates. Standards(NSO, Q19-S644) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kininogen-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Kininogen-1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Kininogen-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Kininogen-1 standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 8.5, Standard deviation: 0.323, CV(%): 3.8
- Sample 2: n=16, Mean(ng/ml): 14, Standard deviation: 0.658, CV(%): 4.7
- Sample 3: n=16, Mean(ng/ml): 28, Standard deviation: 1.54, CV(%): 5.5,
- Sample 1: n=24, Mean(ng/ml): 10, Standard deviation: 0.43, CV(%): 4.3
- Sample 2: n=24, Mean(ng/ml): 19, Standard deviation: 0.969, CV(%): 5.1
- Sample 3: n=24, Mean(ng/ml): 35, Standard deviation: 2.17, CV(%): 6.2
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all KNG1 ELISA试剂盒
- KNG1 (Kininogen 1 (KNG1))
- 别名
- KNG1 (KNG1 产品)
- 别名
- BDK ELISA Kit, BK ELISA Kit, KNG ELISA Kit, Kng ELISA Kit, KNG2 ELISA Kit, fb64g01 ELISA Kit, wu:fb64g01 ELISA Kit, zgc:103569 ELISA Kit, kininogen ELISA Kit, bdk ELISA Kit, kng ELISA Kit, KNG1 ELISA Kit, IHRP ELISA Kit, KINKG ELISA Kit, KINKH ELISA Kit, Kng1 ELISA Kit, Kngk ELISA Kit, kininogen 1 ELISA Kit, kininogen 1 L homeolog ELISA Kit, inter-alpha-trypsin inhibitor heavy chain family member 4 ELISA Kit, kininogen 2-like 1 ELISA Kit, KNG1 ELISA Kit, Kng1 ELISA Kit, kng1 ELISA Kit, kng1.L ELISA Kit, ITIH4 ELISA Kit, Kng2l1 ELISA Kit
- 背景
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Protein Function: (1) Kininogens are inhibitors of thiol proteases, (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII, (3) HMW-kininogen inhibits the thrombin- and plasmin- induced aggregation of thrombocytes, (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action), (5) LMW-kininogen inhibits the aggregation of thrombocytes, (6) LMW- kininogen is in contrast to HMW-kininogen not involved in blood clotting.
Background: Kininogen-1 (KNG1), also known as alpha-2-thiol proteinase inhibitor, Williams-Fitzgerald-Flaujeac factor or the HMWK-kallikrein factor, is a protein that in humans is encoded by the KNG1 gene. It is mapped to 3q27.3. The KNG1 gene uses alternative splicing to generate two different proteins â€" high â€" molecular - weight kininogen (HMWK) and low - molecular- weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. In addition to that, KNG1 is a constituent of the blood coagulation system as well as the kinin-kallikrein system.
Synonyms: Kininogen-1,Alpha-2-thiol proteinase inhibitor,Fitzgerald factor,High molecular weight kininogen,HMWK,Williams-Fitzgerald-Flaujeac factor,Kininogen-1 heavy chain,T-kinin,Ile-Ser-Bradykinin,Bradykinin,Kallidin I,Lysyl-bradykinin,Kallidin II,Kininogen-1 light chain,Low molecular weight growth-promoting factor,KNG1,BDK, KNG,
Full Gene Name: Kininogen-1
Cellular Localisation: Secreted, extracellular space. - 基因ID
- 3827
- UniProt
- P01042
- 途径
- ACE Inhibitor Pathway, Glycosaminoglycan Metabolic Process
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