IL17D ELISA 试剂盒
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- 抗原 See all IL17D ELISA试剂盒
- IL17D (Interleukin 17D (IL17D))
- 抗原表位
- AA 25-205
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-17D
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: A25-R205 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: A25-R205 - Featured
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- Discover our top product IL17D ELISA Kit
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Interleukin 17D (IL17D) ELISA Kit
IL17D 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 17D (IL17D) ELISA KitIL17D 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 17D (IL17D) ELISA KitIL17D 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- mouse IL-17D ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17D has been precoated onto 96-well plates. Standards(E.coli, A25-R205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-17D amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse IL-17D standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-17D standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 715, Standard deviation: 30.03, CV(%): 4.2
- Sample 2: n=16, Mean(pg/ml): 1410, Standard deviation: 64.86, CV(%): 4.6
- Sample 3: n=16, Mean(pg/ml): 2703, Standard deviation: 137.9, CV(%): 5.1,
- Sample 1: n=24, Mean(pg/ml): 694, Standard deviation: 38.17, CV(%): 5.5
- Sample 2: n=24, Mean(pg/ml): 1582, Standard deviation: 99.67, CV(%): 6.3
- Sample 3: n=24, Mean(pg/ml): 2691, Standard deviation: 180.3, CV(%): 6.7
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all IL17D ELISA试剂盒
- IL17D (Interleukin 17D (IL17D))
- 别名
- IL17D (IL17D 产品)
- 别名
- IL-17D ELISA Kit, IL-22 ELISA Kit, IL-27 ELISA Kit, IL27 ELISA Kit, AI462269 ELISA Kit, Il27a ELISA Kit, il17-4 ELISA Kit, zgc:194257 ELISA Kit, zgc:194303 ELISA Kit, IL-27p28 ELISA Kit, Il30 ELISA Kit, p28 ELISA Kit, interleukin 17D ELISA Kit, interleukin 17d ELISA Kit, interleukin 27 ELISA Kit, IL17D ELISA Kit, Il17d ELISA Kit, il17d ELISA Kit, Il27 ELISA Kit
- 背景
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Background: Interleukin 17D (IL-17D) is a member of the unique IL-17 family, which share a conserved cysteine motif. It is mapped to 13q12.11. IL-17D regulates cytokine production in endothelial cells and inhibits hemopoiesis in vitro. Purified recombinant IL-17D inhibited myeloid progenitor cell colony formation. IL-17D can enhance endothelial cell production of IL6, IL8, and GMCSF, but not other cytokines. And it is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas.
Synonyms: IL-17D ,Il17d ,
Full Gene Name: interleukin 17D
- 基因ID
- 239114
- UniProt
- Q8K4C4
- 途径
- Cellular Response to Molecule of Bacterial Origin
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