CSF3R ELISA 试剂盒
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- 抗原 See all CSF3R ELISA试剂盒
- CSF3R (Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R))
- 抗原表位
- AA 25-621
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CSF3R/G-CSF R
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
- NSO, E25-P621
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: E25-P621 - Featured
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- Discover our top product CSF3R ELISA Kit
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Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R) ELISA Kit
CSF3R 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R) ELISA KitCSF3R 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R) ELISA KitCSF3R 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R) ELISA KitCSF3R 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: One or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U-937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U-937 cells. Isoform GCSFR-3 is highly expressed in placenta.
- 板类型
- Pre-coated
- 实验流程
- human CSF3R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CSF3R has been precoated onto 96-well plates. Standards(NSO, E25-P621) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CSF3R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CSF3R amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CSF3R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CSF3R standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 236, Standard deviation: 12.51, CV(%): 5.3
- Sample 2: n=16, Mean(pg/ml): 819, Standard deviation: 50, CV(%): 6.1
- Sample 3: n=16, Mean(pg/ml): 1237, Standard deviation: 59.4, CV(%): 4.8,
- Sample 1: n=24, Mean(pg/ml): 326, Standard deviation: 21.19, CV(%): 6.5
- Sample 2: n=24, Mean(pg/ml): 928, Standard deviation: 66.82, CV(%): 7.2
- Sample 3: n=24, Mean(pg/ml): 1528, Standard deviation: 84.04, CV(%): 5.5
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all CSF3R ELISA试剂盒
- CSF3R (Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R))
- 别名
- CSF3R (CSF3R 产品)
- 别名
- CD114 ELISA Kit, GCSFR ELISA Kit, Cd114 ELISA Kit, Csfgr ELISA Kit, G-CSF-R ELISA Kit, G-CSFR ELISA Kit, CSF3R ELISA Kit, gcsfr ELISA Kit, cd114 ELISA Kit, colony stimulating factor 3 receptor ELISA Kit, colony stimulating factor 3 receptor (granulocyte) ELISA Kit, colony stimulating factor 3 receptor L homeolog ELISA Kit, granulocyte colony-stimulating factor receptor ELISA Kit, CSF3R ELISA Kit, Csf3r ELISA Kit, csf3r ELISA Kit, csf3r.L ELISA Kit, LOC100433338 ELISA Kit
- 背景
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Protein Function: Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface. .
Background: The granulocyte colony-stimulating factor receptor (G-CSF-R), also known as CD114 (Cluster of Differentiation 114), is a protein that in humans is encoded by the CSF3R gene. It is mapped to 1p35-p34.3. G-CSF-R is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF). And the G-CSF-R is a transmembrane receptor that consists of an extracellular ligand-binding portion, a transmembrane domain, and the cytoplasmic portion that is responsible for signal transduction. In addition, GCSF-R ligand-binding is associated with dimerization of the receptor and signal transduction through proteins including Jak, Lyn, STAT, andErk1/2.
Synonyms: Granulocyte colony-stimulating factor receptor,G-CSF receptor,G-CSF-R,CD114,CSF3R,GCSFR,
Full Gene Name: Granulocyte colony-stimulating factor receptor
Cellular Localisation: Isoform 2: Secreted. - 基因ID
- 1441
- UniProt
- Q99062
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