EpCAM ELISA 试剂盒
-
- 抗原 See all EpCAM (EPCAM) ELISA试剂盒
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- 抗原表位
- AA 24-265
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 93.7-6000 pg/mL
- 最低检测浓度
- 93.7 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human EPCAM
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: Q24 - K265 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: Q24-K265 - Featured
- Discover our best selling EPCAM ELISA Kit
- Top Product
- Discover our top product EPCAM ELISA Kit
-
Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit
EPCAM 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epithelial Cell Adhesion Molecule (EPCAM) ELISA KitEPCAM 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Epithelial Cell Adhesion Molecule (EPCAM) ELISA KitEPCAM 适用: 人 Colorimetric Sandwich ELISA 93.7 pg/mL - 6000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
-
- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
-
Sequence similarities: Belongs to the EPCAM family. Contains 1 thyroglobulin type-1 domain.
Tissue Specificity: Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .
- 板类型
- Pre-coated
- 实验流程
- human EPCAM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EPCAM has been precoated onto 96-well plates. Standards(NSO, Q24 - K265) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPCAM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human EPCAM amount of sample captured in plate.
- 实验流程
-
Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m human EPCAM standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human EPCAM standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 475, Standard deviation: 16.15, CV(%): 3.4
- Sample 2: n=16, Mean(pg/ml): 1837, Standard deviation: 77.15, CV(%): 4.2
- Sample 3: n=16, Mean(pg/ml): 3642, Standard deviation: 193.03, CV(%): 5.3,
- Sample 1: n=24, Mean(pg/ml): 531, Standard deviation: 24.43, CV(%): 4.6
- Sample 2: n=24, Mean(pg/ml): 2400, Standard deviation: 136.8, CV(%): 5.7
- Sample 3: n=24, Mean(pg/ml): 4583, Standard deviation: 297.9, CV(%): 6.5
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all EpCAM (EPCAM) ELISA试剂盒
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- 别名
- EPCAM (EPCAM 产品)
- 别名
- DIAR5 ELISA Kit, EGP-2 ELISA Kit, EGP314 ELISA Kit, EGP40 ELISA Kit, ESA ELISA Kit, HNPCC8 ELISA Kit, KS1/4 ELISA Kit, KSA ELISA Kit, M4S1 ELISA Kit, MIC18 ELISA Kit, MK-1 ELISA Kit, TACSTD1 ELISA Kit, TROP1 ELISA Kit, ECS-1 ELISA Kit, ECS1 ELISA Kit, ESA1 ELISA Kit, M17S1 ELISA Kit, EGP ELISA Kit, Ep-CAM ELISA Kit, sb:cb6 ELISA Kit, tacstd ELISA Kit, wu:fj17g02 ELISA Kit, zgc:110304 ELISA Kit, zgc:77119 ELISA Kit, tacstd1 ELISA Kit, MGC80540 ELISA Kit, EPCAM ELISA Kit, egp ELISA Kit, ksa ELISA Kit, m4s1 ELISA Kit, mk-1 ELISA Kit, cd326 ELISA Kit, egp40 ELISA Kit, mic18 ELISA Kit, trop1 ELISA Kit, ep-cam ELISA Kit, hegp-2 ELISA Kit, co17-1a ELISA Kit, ga733-2 ELISA Kit, CD326 ELISA Kit, Egp314 ELISA Kit, EpCAM1 ELISA Kit, GA733-2 ELISA Kit, Ly74 ELISA Kit, Tacsd1 ELISA Kit, Tacstd1 ELISA Kit, gp40 ELISA Kit, epithelial cell adhesion molecule ELISA Kit, flotillin 2 ELISA Kit, epithelial cell adhesion molecule S homeolog ELISA Kit, EPCAM ELISA Kit, FLOT2 ELISA Kit, epcam ELISA Kit, epcam.S ELISA Kit, Epcam ELISA Kit
- 背景
-
Protein Function: May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. .
Background: Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. Also it is involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since it is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.
Synonyms: Epithelial cell adhesion molecule,Ep-CAM,Adenocarcinoma-associated antigen,Cell surface glycoprotein Trop-1,Epithelial cell surface antigen,Epithelial glycoprotein,EGP,Epithelial glycoprotein 314,EGP314,hEGP314,KS 1/4 antigen,KSA,Major gastrointestinal tumor-associated protein GA733-2,Tumor-associated calcium signal transducer 1,CD326,EPCAM,GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1,
Full Gene Name: Epithelial cell adhesion molecule
Cellular Localisation: Lateral cell membrane, Single-pass type I membrane protein . Cell junction, tight junction . Colocalizes with CLDN7 at the lateral cell membrane and tight junction.. - 基因ID
- 4072
- UniProt
- P16422
-
