CXCL9 ELISA 试剂盒
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- 抗原 See all CXCL9 ELISA试剂盒
- CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))
- 抗原表位
- AA 22-126
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CXCL9
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
- E.coli, T22-T126
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: T22-T126 - Featured
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gamma-Interferon-Induced Monokine (CXCL9) ELISA Kit
CXCL9 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
gamma-Interferon-Induced Monokine (CXCL9) ELISA KitCXCL9 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
gamma-Interferon-Induced Monokine (CXCL9) ELISA KitCXCL9 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
gamma-Interferon-Induced Monokine (CXCL9) ELISA KitCXCL9 适用: Cow Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
gamma-Interferon-Induced Monokine (CXCL9) ELISA KitCXCL9 适用: 人 AA 23-125 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
gamma-Interferon-Induced Monokine (CXCL9) ELISA KitCXCL9 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
gamma-Interferon-Induced Monokine (CXCL9) ELISA KitCXCL9 适用: Pig Colorimetric Sandwich ELISA 5-1.400 pg/mL Cell Culture Supernatant, Plasma, Serum
gamma-Interferon-Induced Monokine (CXCL9) ELISA KitCXCL9 适用: 人 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- mouse CXCL9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL9 has been precoated onto 96-well plates. Standards(E.coli, T22-T126) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL9 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CXCL9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CXCL9 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 113, Standard deviation: 4.86, CV(%): 4.3
- Sample 2: n=16, Mean(pg/ml): 468, Standard deviation: 22.00, CV(%): 4.7
- Sample 3: n=16, Mean(pg/ml): 1221, Standard deviation: 69.60, CV(%): 5.7,
- Sample 1: n=24, Mean(pg/ml): 126, Standard deviation: 10.46, CV(%): 8.3
- Sample 2: n=24, Mean(pg/ml): 471, Standard deviation: 41.92, CV(%): 8.9
- Sample 3: n=24, Mean(pg/ml): 1288, Standard deviation: 117.21, CV(%): 9.1
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all CXCL9 ELISA试剂盒
- CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))
- 别名
- CXCL9 (CXCL9 产品)
- 别名
- CMK ELISA Kit, Humig ELISA Kit, MIG ELISA Kit, SCYB9 ELISA Kit, crg-10 ELISA Kit, BB139920 ELISA Kit, Mig ELISA Kit, MuMIG ELISA Kit, Scyb9 ELISA Kit, C-X-C motif chemokine ligand 9 ELISA Kit, chemokine (C-X-C motif) ligand 9 ELISA Kit, CXCL9 ELISA Kit, Cxcl9 ELISA Kit
- 背景
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Protein Function: May be a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response.
Background: Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-i³. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. The standard product used in this kit is recombinant human CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.
Synonyms: C-X-C motif chemokine 9,Gamma-interferon-induced monokine,Monokine induced by interferon-gamma,MIG,MuMIG,Protein m119,Small-inducible cytokine B9,Cxcl9,Mig, Scyb9,
Full Gene Name: C-X-C motif chemokine 9
Cellular Localisation: Secreted. - 基因ID
- 17329
- UniProt
- P18340
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