IGFBP4 ELISA 试剂盒
-
- 抗原 See all IGFBP4 ELISA试剂盒
- IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))
- 抗原表位
- AA 22-258
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 500-32000 pg/mL
- 最低检测浓度
- 500 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGFBP4
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
- NSO, D22-E258
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <20pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: D22-E258 - Featured
- Discover our best selling IGFBP4 ELISA Kit
- Top Product
- Discover our top product IGFBP4 ELISA Kit
-
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA Kit
IGFBP4 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 适用: 小鸡 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 适用: Cow Colorimetric Competition ELISA 74.07 pg/mL - 6000 pg/mL Plasma, Serum
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 适用: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 适用: 人 Colorimetric Sandwich ELISA 500 pg/mL - 32000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 适用: 山羊 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- human IGFBP4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP4 has been precoated onto 96-well plates. Standards (NSO, D22-E258) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP4 amount of sample captured in plate.
- 实验流程
-
Aliquot 0.1 mL per well of the 32000pg/mL, 16000pg/mL, 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL human IGFBP4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGFBP4 standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 846, Standard deviation: 43.99, CV(%): 5.2
- Sample 2: n=16, Mean(pg/ml): 3152, Standard deviation: 179.66, CV(%): 5.7
- Sample 3: n=16, Mean(pg/ml): 12519, Standard deviation: 688.54, CV(%): 5.5,
- Sample 1: n=24, Mean(pg/ml): 755, Standard deviation: 49.83, CV(%): 6.6
- Sample 2: n=24, Mean(pg/ml): 3646, Standard deviation: 258.86, CV(%): 7.1
- Sample 3: n=24, Mean(pg/ml): 13698, Standard deviation: 876.67, CV(%): 6.4
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all IGFBP4 ELISA试剂盒
- IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))
- 别名
- IGFBP4 (IGFBP4 产品)
- 别名
- IGFBP4 ELISA Kit, igfbp4 ELISA Kit, BP-4 ELISA Kit, HT29-IGFBP ELISA Kit, IBP4 ELISA Kit, IGFBP-4 ELISA Kit, AI875747 ELISA Kit, Deb2 ELISA Kit, IGF-BP4 ELISA Kit, IBP-4 ELISA Kit, insulin like growth factor binding protein 4 ELISA Kit, insulin-like growth factor-binding protein 4 ELISA Kit, insulin-like growth factor binding protein 4 ELISA Kit, IGFBP4 ELISA Kit, LOC100136305 ELISA Kit, LOC100194498 ELISA Kit, igfbp4 ELISA Kit, Igfbp4 ELISA Kit
- 背景
-
Protein Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Background: Insulin-like growth factor-binding protein 4 is a protein that in humans is encoded by the IGFBP4gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. In addition, IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. It is secreted by all colon cancer cells.
Synonyms: Insulin-like growth factor-binding protein 4,IBP-4,IGF-binding protein 4,IGFBP-4,IGFBP4,IBP4,
Full Gene Name: Insulin-like growth factor-binding protein 4
Cellular Localisation: Secreted. - 基因ID
- 3487
- UniProt
- P22692
- 途径
- WNT signaling, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process
-
