NrCAM ELISA 试剂盒

Tissue Specificity: Detected in all the examined tissues. In the brain it was detected in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus.

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human NRCAM standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human NRCAM standard solution and each sample be measured in duplicate.

• Sample 1: n=16, Mean(pg/ml): 675, Standard deviation: 35.10, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 1308, Standard deviation: 79.78, CV(%): 6.1
• Sample 3: n=16, Mean(pg/ml): 2147, Standard deviation: 124.52, CV(%): 5.8,
• Sample 1: n=24, Mean(pg/ml): 694, Standard deviation: 40.25, CV(%): 5.8
• Sample 2: n=24, Mean(pg/ml): 1382, Standard deviation: 89.83, CV(%): 6.5
• Sample 3: n=24, Mean(pg/ml): 2503, Standard deviation: 155.18, CV(%): 6.2

Protein Function: Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding. Plays a role in mediating cell-cell contacts between Schwann cells and axons. Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes, not required for the formation of mature nodes with normal sodium channel clusters. Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier. .

Background: Neuronal cell adhesion molecule is a protein that in humans is encoded by the NRCAM gene. Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. Also, this gene is expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms.

Synonyms: Neuronal cell adhesion molecule,Nr-CAM,Neuronal surface protein Bravo ,hBravo ,NgCAM-related cell adhesion molecule,Ng-CAM-related,NRCAM,KIAA0343,

Full Gene Name: Neuronal cell adhesion molecule