COMT ELISA 试剂盒
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- 抗原 See all COMT ELISA试剂盒
- COMT (Catechol-O-Methyltransferase (COMT))
- 抗原表位
- AA 52-271
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human COMT
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
- E.coli, G52-P271
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: G52-P271 - Featured
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Catechol-O-Methyltransferase (COMT) ELISA Kit
COMT 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catechol-O-Methyltransferase (COMT) ELISA KitCOMT 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Catechol-O-Methyltransferase (COMT) ELISA KitCOMT 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Catechol-O-Methyltransferase (COMT) ELISA KitCOMT 适用: 山羊 Colorimetric Competition ELISA Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Catechol-O-Methyltransferase (COMT) ELISA KitCOMT 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Brain, liver, placenta, lymphocytes and erythrocytes.
- 板类型
- Pre-coated
- 实验流程
- human COMT ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for COMT has been precoated onto 96-well plates. Standards (E.coli, G52-P271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for COMT is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human COMT amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human COMT standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human COMT standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 305, Standard deviation: 15.55, CV(%): 5.1
- Sample 2: n=16, Mean(pg/ml): 818, Standard deviation: 43.35, CV(%): 5.3
- Sample 3: n=16, Mean(pg/ml): 1249, Standard deviation: 77.43, CV(%): 6.2,
- Sample 1: n=24, Mean(pg/ml): 254, Standard deviation: 16.25, CV(%): 6.4
- Sample 2: n=24, Mean(pg/ml): 699, Standard deviation: 38.44, CV(%): 5.5
- Sample 3: n=24, Mean(pg/ml): 1358, Standard deviation: 92.34, CV(%): 6.8
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all COMT ELISA试剂盒
- COMT (Catechol-O-Methyltransferase (COMT))
- 别名
- COMT (COMT 产品)
- 别名
- Comt1 ELISA Kit, D16Wsu103e ELISA Kit, D330014B15Rik ELISA Kit, COMT ELISA Kit, mb-comt ELISA Kit, s-comt ELISA Kit, comt ELISA Kit, MGC154444 ELISA Kit, zgc:114157 ELISA Kit, si:dkey-13a21.15 ELISA Kit, zK13A21.15 ELISA Kit, zgc:162236 ELISA Kit, catechol-O-methyltransferase ELISA Kit, microRNA 4761 ELISA Kit, catechol-O-methyltransferase S homeolog ELISA Kit, methyltransferase ELISA Kit, catechol-o-methyltransferase ELISA Kit, catechol-O-methyltransferase a ELISA Kit, catechol-O-methyltransferase b ELISA Kit, catechol O-methyltransferase ELISA Kit, COMT ELISA Kit, Comt ELISA Kit, MIR4761 ELISA Kit, comt ELISA Kit, comt.2 ELISA Kit, comt.S ELISA Kit, Rv1703c ELISA Kit, Mb1729c ELISA Kit, comta ELISA Kit, comtb ELISA Kit, LOC100354142 ELISA Kit
- 背景
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Protein Function: Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
Background: Catechol-O-methyltransferase (COMT) is one of several enzymes that degrade catecholamines such as dopamine, epinephrine, and norepinephrine. In humans, catechol-O-methyltransferase protein is encoded by the COMT gene. As the regulation of catecholamines is impaired in a number of medical conditions, several pharmaceutical drugs target COMT to alter its activity and therefore the availability of catecholamines. In the brain, COMT-dependent dopamine degradation is of particular importance in brain regions with low expression of the presynaptic dopamine transporter (DAT), such as the prefrontal cortex. This process is supposed to take place in postsynaptic neurons, as, in general, COMT is located intracellularly in the central nervous system (CNS).
Synonyms: Catechol O-methyltransferase,2.1.1.6,COMT,
Full Gene Name: Catechol O-methyltransferase
Cellular Localisation: Isoform Soluble: Cytoplasm. - 基因ID
- 1312
- UniProt
- P21964
- 途径
- Steroid Hormone Biosynthesis, SARS-CoV-2 Protein Interactome
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