TDGF1 ELISA 试剂盒
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- 抗原 See all TDGF1 ELISA试剂盒
- TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))
- 抗原表位
- AA 26-150
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Cripto/TDGF1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
- NSO, R26-Q150
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: R26-Q150 - Featured
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Teratocarcinoma-Derived Growth Factor 1 (TDGF1) ELISA Kit
TDGF1 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Teratocarcinoma-Derived Growth Factor 1 (TDGF1) ELISA KitTDGF1 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Teratocarcinoma-Derived Growth Factor 1 (TDGF1) ELISA KitTDGF1 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Expressed at low level in specific organs of the adult animal such as spleen, heart, lung and brain. During gastrulation, expressed in the forming mesoderm. In later stages of the developing heart, expression is restricted to the truncus arteriosus.
- 板类型
- Pre-coated
- 实验流程
- mouse Cripto ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cripto has been precoated onto 96-well plates. Standards (NSO, R26-Q150) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cripto is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Cripto amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Cripto standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Cripto standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 675, Standard deviation: 34.42, CV(%): 5.1
- Sample 2: n=16, Mean(pg/ml): 1810, Standard deviation: 108.6, CV(%): 6.0
- Sample 3: n=16, Mean(pg/ml): 2944, Standard deviation: 197.24, CV(%): 6.7,
- Sample 1: n=24, Mean(pg/ml): 792, Standard deviation: 43.56, CV(%): 5.5
- Sample 2: n=24, Mean(pg/ml): 1948, Standard deviation: 140.25, CV(%): 7.2
- Sample 3: n=24, Mean(pg/ml): 3107, Standard deviation: 211.27, CV(%): 6.8
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all TDGF1 ELISA试剂盒
- TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))
- 别名
- TDGF1 (TDGF1 产品)
- 别名
- CR ELISA Kit, CRGF ELISA Kit, CRIPTO ELISA Kit, CR1 ELISA Kit, cripto ELISA Kit, cb85 ELISA Kit, tdgf1 ELISA Kit, wu:fe01d04 ELISA Kit, zgc:109829 ELISA Kit, Cripto-1 ELISA Kit, cr1 ELISA Kit, cr1a ELISA Kit, frl-1 ELISA Kit, frl1 ELISA Kit, xcr1 ELISA Kit, TDGF1 ELISA Kit, tdgf3 ELISA Kit, cripto-3 ELISA Kit, teratocarcinoma-derived growth factor 1 ELISA Kit, teratocarcinoma-derived growth factor 1, member 3 L homeolog ELISA Kit, teratocarcinoma-derived growth factor 1, member 1 ELISA Kit, TDGF1 ELISA Kit, Tdgf1 ELISA Kit, tdgf1 ELISA Kit, tdgf1.3.L ELISA Kit, tdgf1.1 ELISA Kit
- 背景
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Protein Function: Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
Background: Teratocarcinoma-derived growth factor 1 is a protein that in humans is encoded by the TDGF1 gene. It is mapped to 3p23-p21. The protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic developmentand tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants.
Synonyms: Teratocarcinoma-derived growth factor,Cripto growth factor,Epidermal growth factor-like Cripto protein,Tdgf1,Cripto,
Full Gene Name: Teratocarcinoma-derived growth factor
Cellular Localisation: Cell membrane, Lipid-anchor, GPI-anchor. - 基因ID
- 21667
- UniProt
- P51865
- 途径
- EGFR Signaling Pathway
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