Stanniocalcin 1 ELISA 试剂盒
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- 抗原 See all Stanniocalcin 1 (STC1) ELISA试剂盒
- Stanniocalcin 1 (STC1)
- 抗原表位
- AA 34-247
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Stanniocalcin 1/STC1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
- NSO, V34-A247
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: V34-A247 - Featured
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Stanniocalcin 1 (STC1) ELISA Kit
STC1 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Stanniocalcin 1 (STC1) ELISA KitSTC1 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Stanniocalcin 1 (STC1) ELISA KitSTC1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Stanniocalcin 1 (STC1) ELISA KitSTC1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Expressed in most tissues, with the highest levels in ovary, prostate, heart, kidney and thyroid. In the kidney, expression is confined to the nephron, specifically in the distal convoluted tubule and in the collecting tubule. Not detected in the brain, liver, spleen, peripheral blood leukocytes and adrenal medulla.
- 板类型
- Pre-coated
- 实验流程
- human STC1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for STC1 has been precoated onto 96-well plates. Standards (NSO, V34-A247) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for STC1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human STC1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human STC1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human STC1 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 246, Standard deviation: 14.02, CV(%): 5.7
- Sample 2: n=16, Mean(pg/ml): 801, Standard deviation: 48.06, CV(%): 6.0
- Sample 3: n=16, Mean(pg/ml): 1351, Standard deviation: 74.30, CV(%): 5.5,
- Sample 1: n=24, Mean(pg/ml): 254, Standard deviation: 15.74, CV(%): 6.2
- Sample 2: n=24, Mean(pg/ml): 775, Standard deviation: 49.6, CV(%): 6.4
- Sample 3: n=24, Mean(pg/ml): 1416, Standard deviation: 67.96, CV(%): 6.8
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all Stanniocalcin 1 (STC1) ELISA试剂盒
- Stanniocalcin 1 (STC1)
- 别名
- STC1 (STC1 产品)
- 别名
- STC ELISA Kit, Stc ELISA Kit, stc1 ELISA Kit, MGC82280 ELISA Kit, STC1 ELISA Kit, zgc:153307 ELISA Kit, LOC777959 ELISA Kit, stanniocalcin 1 ELISA Kit, stanniocalcin 1 L homeolog ELISA Kit, stanniocalcin 1 S homeolog ELISA Kit, STC1 ELISA Kit, Stc1 ELISA Kit, stc1.L ELISA Kit, stc1.S ELISA Kit, stc1 ELISA Kit, LOC777959 ELISA Kit
- 背景
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Protein Function: Stimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia.
Background: Stanniocalcin-1 is a glycoprotein which is encoded by the STC1 gene. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. Human Stanniocalcin-1 is a putative molecular biomarker of leukemic microenvironment and the only molecular function known up to date is a SUMO E3 ligase activity in the SUMOylation cycle. STC1 interacts with lots of proteins in the cytoplasm, mitochondria, endoplasmatic reticulum and dot-like fashion in the nucleus. The N-terminal region of STC1 is the function region which is responsible to establish the interaction with its partners, including SUMO1. Low resolution studies shows that STC1 is an anti-parallel homodimer in solution and the cystein 202 is responsible for the dimerization of this protein. All the 5 dissulfide bonds of human STC1 are conserved and have the same profile of fish STC.
Synonyms: Stanniocalcin-1,STC-1,STC1,STC,
Full Gene Name: Stanniocalcin-1
Cellular Localisation: Secreted. - 基因ID
- 6781
- UniProt
- P52823
- 途径
- Hormone Activity
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