SERPINC1 ELISA 试剂盒
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- 抗原 See all SERPINC1 ELISA试剂盒
- SERPINC1 (Serpin Family C Member 1 (SERPINC1))
- 抗原表位
- AA 33-464
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 312-20.000 pg/mL
- 最低检测浓度
- 312 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Serpin C1/Antithrombin-III
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine
- Analytical Method
- Quantitative
- 特异性
- NSO, H33-K464
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: H33-K464 - Featured
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Serpin Family C Member 1 (SERPINC1) ELISA Kit
SERPINC1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Plasma
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 人 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 大鼠 Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 人 Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Cell Culture Supernatant, Plasma, Serum
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 小鼠 Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Plasma, Serum
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 大鼠 Colorimetric Sandwich ELISA 39 ng/mL - 2500 ng/mL Plasma, Serum, Tissue Homogenate
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 小鼠 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 小鼠 Colorimetric Sandwich ELISA 0.937 ng/mL - 60 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 人 Colorimetric Sandwich ELISA 3.125-200 ng/mL Cell Culture Cells, Cerebrospinal Fluid, Milk, Saliva, Urine
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Found in plasma.
- 板类型
- Pre-coated
- 实验流程
- human Serpin C1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Serpin C1 has been precoated onto 96-well plates. Standards (NSO, H33-K464) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Serpin C1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Serpin C1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Serpin C1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA, citrate) or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Serpin C1 standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.05, Standard deviation: 0.064, CV(%): 6.1
- Sample 2: n=16, Mean(ng/ml): 3.42, Standard deviation: 0.194, CV(%): 5.7
- Sample 3: n=16, Mean(ng/ml): 8.67, Standard deviation: 0.546, CV(%): 6.3,
- Sample 1: n=24, Mean(ng/ml): 1.32, Standard deviation: 0.088, CV(%): 6.7
- Sample 2: n=24, Mean(ng/ml): 3.74, Standard deviation: 0.239, CV(%): 6.4
- Sample 3: n=24, Mean(ng/ml): 8.23, Standard deviation: 0.543, CV(%): 6.6
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all SERPINC1 ELISA试剂盒
- SERPINC1 (Serpin Family C Member 1 (SERPINC1))
- 别名
- SERPINC1 (SERPINC1 产品)
- 别名
- AT3 ELISA Kit, AT3D ELISA Kit, ATIII ELISA Kit, THPH7 ELISA Kit, SERPINC1 ELISA Kit, DKFZp470C1733 ELISA Kit, AI114908 ELISA Kit, At-3 ELISA Kit, At3 ELISA Kit, ANTITHROMBIN, AT-III ELISA Kit, AT-III ELISA Kit, serpin family C member 1 ELISA Kit, antithrombin-III ELISA Kit, serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 ELISA Kit, SERPINC1 ELISA Kit, CpipJ_CPIJ000472 ELISA Kit, CpipJ_CPIJ013111 ELISA Kit, Serpinc1 ELISA Kit
- 背景
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Protein Function: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. .
Background: Antithrombin III is the most important inhibitor of thrombin and other coagulation proteinases. It belongs to the serine proteinase inhibitor (serpin) superfamily of inhibitors and structurally related proteins, which contain reactive centers that have evolved to attract and entrap certain proteinases. Inherited antithrombin III deficiency (AT3D) is a risk factor for the early development of venous thromboembolism (THPH7). Antithrombin III regulates clot formation both by inhibiting thrombin activity directly and by interfering with earlier stages of the clotting cascade. By in situ hybridization and quantitative analysis of DNA dosage in carriers of chromosome 1 deletions, AT3 is mapped to 1q23-q25. The AT3 gene has 7 exons. It contains 9 complete and 1 partial repetitive ALU sequence elements, which occur in the introns of the gene at a higher frequency (about 22 % of the intron sequence) than in the genome as a whole (about 5 %).
Synonyms: Antithrombin-III,ATIII,Serpin C1,SERPINC1,AT3,PRO0309,
Full Gene Name: Antithrombin-III
Cellular Localisation: Secreted, extracellular space. - 基因ID
- 462
- UniProt
- P01008
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