LOXL2 ELISA 试剂盒

Tissue Specificity: Expressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate. Up- regulated in a number of cancers cells and tissues.

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human LOXL2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human LOXL2 standard solution and each sample be measured in duplicate.

• Sample 1: n=16, Mean(pg/ml): 1589, Standard deviation: 79.45, CV(%): 5.0
• Sample 2: n=16, Mean(pg/ml): 3122, Standard deviation: 171.71, CV(%): 5.5
• Sample 3: n=16, Mean(pg/ml): 7510, Standard deviation: 435.58, CV(%): 5.8,
• Sample 1: n=24, Mean(pg/ml): 1466, Standard deviation: 76.23, CV(%): 5.2
• Sample 2: n=24, Mean(pg/ml): 4236, Standard deviation: 258.39, CV(%): 6.1
• Sample 3: n=24, Mean(pg/ml): 6998, Standard deviation: 447.87, CV(%): 6.4

Protein Function: Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When nuclear, acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E- cadherin, probably by mediating deamination of histone H3. Also involved in E-cadherin repression following hypoxia, a hallmark of epithelial to mesenchymal transition believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression. Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation. .

Background: Lysyl oxidase homolog 2 is an enzyme that in humans is encoded by the LOXL2 gene. This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. LOXL2 can also crosslink collagen type IV and hence influence the sprouting of new blood vessels.

Synonyms: Lysyl oxidase homolog 2,1.4.3.13,Lysyl oxidase-like protein 2,Lysyl oxidase-related protein 2,Lysyl oxidase-related protein WS9-14,LOXL2,

Full Gene Name: Lysyl oxidase homolog 2