FGF21 ELISA 试剂盒
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- 抗原 See all FGF21 ELISA试剂盒
- FGF21 (Fibroblast Growth Factor 21 (FGF21))
- 抗原表位
- AA 29-210
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse FGF21
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
- E.coli, A29-S210
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: A29-S210 - Featured
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- Discover our top product FGF21 ELISA Kit
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Fibroblast Growth Factor 21 (FGF21) ELISA Kit
FGF21 适用: 小鼠 Colorimetric Competition ELISA 12.5 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 21 (FGF21) ELISA KitFGF21 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 21 (FGF21) ELISA KitFGF21 适用: 大鼠 Colorimetric Competition ELISA 12.5 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 21 (FGF21) ELISA KitFGF21 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 21 (FGF21) ELISA KitHigh Sensitivity FGF21 适用: 小鼠 Colorimetric Competition ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 21 (FGF21) ELISA KitFGF21 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Fibroblast Growth Factor 21 (FGF21) ELISA KitFGF21 适用: Cow Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 21 (FGF21) ELISA KitFGF21 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 21 (FGF21) ELISA KitWide Range FGF21 适用: 大鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 21 (FGF21) ELISA KitFGF21 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Most abundantly expressed in the liver, also expressed in the thymus at lower levels. .
- 板类型
- Pre-coated
- 实验流程
- mouse FGF21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FGF21 has been precoated onto 96-well plates. Standards(E.coli,A29-S210) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse FGF21 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse FGF21 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse FGF21 standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 245, Standard deviation: 11.51, CV(%): 4.7
- Sample 2: n=16, Mean(pg/ml): 656, Standard deviation: 28.20, CV(%): 4.3
- Sample 3: n=16, Mean(pg/ml): 1422, Standard deviation: 71.1, CV(%): 5.0,
- Sample 1: n=24, Mean(pg/ml): 210, Standard deviation: 12.81, CV(%): 6.1
- Sample 2: n=24, Mean(pg/ml): 670, Standard deviation: 43.55, CV(%): 6.5
- Sample 3: n=24, Mean(pg/ml): 1572, Standard deviation: 99.03, CV(%): 6.3
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy." in: Scientific reports, Vol. 5, pp. 11219, (2015) (PubMed).
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: "Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy." in: Scientific reports, Vol. 5, pp. 11219, (2015) (PubMed).
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- 抗原 See all FGF21 ELISA试剂盒
- FGF21 (Fibroblast Growth Factor 21 (FGF21))
- 别名
- FGF21 (FGF21 产品)
- 别名
- FGF21 ELISA Kit, fibroblast growth factor 21 ELISA Kit, FGF21 ELISA Kit, fgf21 ELISA Kit, Fgf21 ELISA Kit
- 背景
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Protein Function: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity probably requires the presence of KLB. .
Background: Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.
Synonyms: Fibroblast growth factor 21,FGF-21,Fgf21,
Full Gene Name: Fibroblast growth factor 21
Cellular Localisation: Secreted. - 基因ID
- 56636
- UniProt
- Q9JJN1
- 途径
- RTK signaling
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