Neuregulin 1 ELISA 试剂盒
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- 抗原 See all Neuregulin 1 (NRG1) ELISA试剂盒
- Neuregulin 1 (NRG1)
- 抗原表位
- AA 2-246
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Neuregulin-1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: S2-K246 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: S2-K246 - Featured
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Neuregulin 1 (NRG1) ELISA Kit
NRG1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Neuregulin 1 (NRG1) ELISA KitNRG1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neuregulin 1 (NRG1) ELISA KitNRG1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neuregulin 1 (NRG1) ELISA KitNRG1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Neuregulin 1 (NRG1) ELISA KitNRG1 适用: 大鼠 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
Neuregulin 1 (NRG1) ELISA KitNRG1 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Neuregulin 1 (NRG1) ELISA KitWide Range NRG1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the neuregulin family.
Tissue Specificity: Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells, in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific. .
- 板类型
- Pre-coated
- 实验流程
- human Neuregulin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Neuregulin-1 has been precoated onto 96-well plates. Standards(E.coli, S2-K246) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neuregulin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Neuregulin-1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Neuregulin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Neuregulin-1 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 675, Standard deviation: 28.35, CV(%): 4.2
- Sample 2: n=16, Mean(pg/ml): 1308, Standard deviation: 61.48, CV(%): 4.7
- Sample 3: n=16, Mean(pg/ml): 2147, Standard deviation: 113.8, CV(%): 5.3,
- Sample 1: n=24, Mean(pg/ml): 694, Standard deviation: 40.25, CV(%): 5.8
- Sample 2: n=24, Mean(pg/ml): 1382, Standard deviation: 84.3, CV(%): 6.1
- Sample 3: n=24, Mean(pg/ml): 2503, Standard deviation: 172.7, CV(%): 6.9
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all Neuregulin 1 (NRG1) ELISA试剂盒
- Neuregulin 1 (NRG1)
- 别名
- NRG1 (NRG1 产品)
- 别名
- 6030402G23Rik ELISA Kit, ARIA ELISA Kit, D230005F13Rik ELISA Kit, GGF ELISA Kit, GGFII ELISA Kit, HRG ELISA Kit, HRGalpha ELISA Kit, Hgl ELISA Kit, NDF ELISA Kit, SMDF ELISA Kit, GGF2 ELISA Kit, HGL ELISA Kit, HRG1 ELISA Kit, HRGA ELISA Kit, MST131 ELISA Kit, NRG-1 ELISA Kit, heregulin ELISA Kit, nrg1-A ELISA Kit, pro-neuregulin-1 ELISA Kit, NRG1 ELISA Kit, wu:fm70e01 ELISA Kit, DKFZp459A1838 ELISA Kit, neuregulin 1 ELISA Kit, histidine rich glycoprotein ELISA Kit, neuregulin 1 L homeolog ELISA Kit, Nrg1 ELISA Kit, NRG1 ELISA Kit, HRG ELISA Kit, nrg1.L ELISA Kit, nrg1 ELISA Kit
- 背景
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Protein Function: Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells, inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction, stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells, stimulating Schwann cell proliferation, implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. .
Background: Neuregulin 1 or NRG1 is a protein that in humans is encoded by the NRG1 gene. It is mapped to 8p12. NRG1 is one of four proteins in the neuregulin family that act on the EGFR family of receptors. The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). It is also essential for the normal development of the nervous system and the heart. Furthermore, it was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues.
Synonyms: Pro-neuregulin-1, membrane-bound isoform,Pro-NRG1,Neuregulin-1,Acetylcholine receptor-inducing activity,ARIA,Breast cancer cell differentiation factor p45,Glial growth factor,Heregulin,HRG,Neu differentiation factor,Sensory and motor neuron-derived factor,NRG1,GGF, HGL, HRGA, NDF, SMDF,
Full Gene Name: Pro-neuregulin-1, membrane-bound isoform
Cellular Localisation: Pro-neuregulin-1, membrane-bound isoform: Cell membrane, Single-pass type I membrane protein. Does not seem to be active. - 基因ID
- 3084
- UniProt
- Q02297
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation
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