ANGPTL3 ELISA 试剂盒
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- 抗原 See all ANGPTL3 ELISA试剂盒
- ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))
- 抗原表位
- AA 17-460
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 312-20.000 pg/mL
- 最低检测浓度
- 312 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ANGPTL3
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: sf21
Immunogen sequence: S17-E460 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: sf21
Immunogen sequence: S17-E460 - Featured
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Angiopoietin-Like 3 (ANGPTL3) ELISA Kit
ANGPTL3 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 3 (ANGPTL3) ELISA KitANGPTL3 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Angiopoietin-Like 3 (ANGPTL3) ELISA KitANGPTL3 适用: 猴 Colorimetric Sandwich ELISA 39.06 pg/mL - 2500 pg/mL Cell Culture Supernatant, Plasma, Serum
Angiopoietin-Like 3 (ANGPTL3) ELISA KitANGPTL3 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 3 (ANGPTL3) ELISA KitANGPTL3 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 3 (ANGPTL3) ELISA KitANGPTL3 适用: 小鼠 AA 17-455 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Contains 1 fibrinogen C-terminal domain.
Tissue Specificity: Expressed principally in liver. Weakly expressed in kidney. .
- 板类型
- Pre-coated
- 实验流程
- human ANGPTL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ANGPTL3 has been precoated onto 96-well plates. Standards(sf21, S17-E460) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANGPTL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ANGPTL3 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human ANGPTL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ANGPTL3 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 3.2, Standard deviation: 0.144, CV(%): 4.5
- Sample 2: n=16, Mean(ng/ml): 5.8, Standard deviation: 0.273, CV(%): 4.7
- Sample 3: n=16, Mean(ng/ml): 10.3, Standard deviation: 0.299, CV(%): 2.9,
- Sample 1: n=24, Mean(ng/ml): 3.5, Standard deviation: 0.238, CV(%): 6.8
- Sample 2: n=24, Mean(ng/ml): 6.2, Standard deviation: 0.459, CV(%): 7.4
- Sample 3: n=24, Mean(ng/ml): 12.1, Standard deviation: 0.762, CV(%): 6.3
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all ANGPTL3 ELISA试剂盒
- ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))
- 别名
- ANGPTL3 (ANGPTL3 产品)
- 别名
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- 背景
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Background: ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.
Synonyms: Angiopoietin-related protein 3,Angiopoietin-5,ANG-5,Angiopoietin-like protein 3,ANGPTL3,ANGPT5,UNQ153/PRO179,
Full Gene Name: Angiopoietin-related protein 3
Cellular Localisation: Secreted. - 基因ID
- 27329
- UniProt
- Q9Y5C1
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