TFPI2 ELISA 试剂盒
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- TFPI2 (Tissue Factor Pathway Inhibitor 2 (TFPI2))
- 抗原表位
- AA 23-235
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10.000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TFPI2
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: D23-F235 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: D23-F235 - Featured
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Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
TFPI2 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA KitTFPI2 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA KitTFPI2 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma
Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA KitTFPI2 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy.
- 板类型
- Pre-coated
- 实验流程
- human TFPI2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TFPI2 has been precoated onto 96-well plates. Standards(NSO, D23-F235) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TFPI2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TFPI2 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TFPI2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TFPI2 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.75, Standard deviation: 0.074, CV(%): 4.2
- Sample 2: n=16, Mean(ng/ml): 3.28, Standard deviation: 0.154, CV(%): 4.7
- Sample 3: n=16, Mean(ng/ml): 6.32, Standard deviation: 0.354, CV(%): 5.6,
- Sample 1: n=24, Mean(ng/ml): 1.64, Standard deviation: 0.08, CV(%): 4.9
- Sample 2: n=24, Mean(ng/ml): 3.09, Standard deviation: 0.164, CV(%): 5.3
- Sample 3: n=24, Mean(ng/ml): 6.47, Standard deviation: 0.395, CV(%): 6.1
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all TFPI2 ELISA试剂盒
- TFPI2 (Tissue Factor Pathway Inhibitor 2 (TFPI2))
- 别名
- TFPI2 (TFPI2 产品)
- 别名
- MGC68843 ELISA Kit, TFPI2 ELISA Kit, MGC108301 ELISA Kit, si:ch211-262k23.2 ELISA Kit, tfpi2 ELISA Kit, PP5 ELISA Kit, REF1 ELISA Kit, TFPI-2 ELISA Kit, AV000670 ELISA Kit, PP5/TFPI-2 ELISA Kit, tissue factor pathway inhibitor 2 L homeolog ELISA Kit, tissue factor pathway inhibitor 2 ELISA Kit, tfpi2.L ELISA Kit, TFPI2 ELISA Kit, tfpi2 ELISA Kit, Tfpi2 ELISA Kit
- 背景
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Protein Function: May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin. .
Background: TFPI2, also called Tissue factor pathway inhibitor 2, is a protein that in humans is encoded by the TFPI2 gene. It is mapped to 7q21.3. This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Qualitative PCR and microarray analyses showed that expression of TFPI2 was inversely correlated with that of MIR616 in prostate cancer cell lines, primary tumors of various grades, and normal tissue.
Synonyms: Tissue factor pathway inhibitor 2,TFPI-2,Placental protein 5,PP5,TFPI2,
Full Gene Name: Tissue factor pathway inhibitor 2
Cellular Localisation: Secreted. - 基因ID
- 7980
- UniProt
- P48307
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