BIRC7 ELISA 试剂盒

Sequence similarities: Belongs to the IAP family.

Tissue Specificity: Isoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes.

Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Livin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, cell lysates or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Livin standard solution and each sample be measured in duplicate.

• Sample 1: n=16, Mean(ng/ml): 8.5, Standard deviation: 0.459, CV(%): 5.4
• Sample 2: n=16, Mean(ng/ml): 19, Standard deviation: 0.874, CV(%): 4.6
• Sample 3: n=16, Mean(ng/ml): 32, Standard deviation: 1.312, CV(%): 4.1,
• Sample 1: n=24, Mean(ng/ml): 10, Standard deviation: 0.72, CV(%): 7.2
• Sample 2: n=24, Mean(ng/ml): 24, Standard deviation: 1.56, CV(%): 6.5
• Sample 3: n=24, Mean(ng/ml): 37, Standard deviation: 2.146, CV(%): 5.8

Protein Function: Cytokine that plays a crucial role in innate immunity of the epithelium, including to intestinal bacterial pathogens, in an autocrine manner. Stimulates the production of antibacterial peptides and proinflammatory molecules for host defense by signaling through the NF-kappa-B and MAPK pathways. Acts synergically with IL22 in inducing the expression of antibacterial peptides, including S100A8, S100A9, REG3A and REG3G. Synergy is also observed with TNF and IL1B in inducing DEFB2 from keratinocytes. Depending on the type of insult, may have both protective and pathogenic properties, either by maintaining epithelial homeostasis after an inflammatory challenge or by promoting inflammatory phenotype. Enhanced IL17C/IL17RE signaling may also lead to greater susceptibility to autoimmune diseases. .

Background: BIRC7, also called KIAP or Livin, is a protein that in humans is encoded by the BIRC7 gene. This gene is mapped to 20q13.33. It encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. BIRC7 blocked apoptosis induced by BAX induction of cytochrome C release from mitochondria, and it also can block apoptosis induced by chemotherapeutic agents., IL17C, also known as CX2, is a protein that in humans is encoded by the IL17C gene. IL17C is mapped to 16q24.3. The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. IL17C is an essential autocrine cytokine regulating innate epithelial immune responses. It also plays an important role in the pathogenesis of inflammatory arthritis.

Synonyms: Interleukin-17C,IL-17C,Cytokine CX2,IL17C,UNQ561/PRO1122, CX2|Cytokine CX2|IL 17C|IL 21|IL-17C|IL17C|IL17C_HUMAN|Interleukin 17C|Interleukin-17C|MGC126884|MGC138401

Full Gene Name: Interleukin-17C, interleukin 17C