FLT4 ELISA 试剂盒
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- 抗原 See all FLT4 ELISA试剂盒
- FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))
- 抗原表位
- AA 25-770
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10.000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat VEGFR3/FLT4
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: Y25-D770 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: Y25-D770 - Featured
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- Discover our top product FLT4 ELISA Kit
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Fms-Related Tyrosine Kinase 4 (FLT4) ELISA Kit
FLT4 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitHigh Sensitivity FLT4 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 小鼠 AA 25-770 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Serum
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 人 Colorimetric Sandwich ELISA 15-3000 pg/mL Cell Culture Supernatant, Plasma, Serum
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 小鼠 Colorimetric Sandwich ELISA 0.2-50 ng/mL Cell Culture Supernatant, Plasma, Serum
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA KitFLT4 适用: 人 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
- 板类型
- Pre-coated
- 实验流程
- rat VEGFR3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VEGFR3 has been precoated onto 96-well plates. Standards(NSO, Y25-D770) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGFR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat VEGFR3 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat VEGFR3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat VEGFR3 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 1580, Standard deviation: 52.14, CV(%): 3.3
- Sample 2: n=16, Mean(pg/ml): 3021, Standard deviation: 142, CV(%): 4.7
- Sample 3: n=16, Mean(pg/ml): 6135, Standard deviation: 319, CV(%): 5.2,
- Sample 1: n=24, Mean(pg/ml): 1749, Standard deviation: 80.45, CV(%): 4.6
- Sample 2: n=24, Mean(pg/ml): 3312, Standard deviation: 182.2, CV(%): 5.5
- Sample 3: n=24, Mean(pg/ml): 6758, Standard deviation: 432.5, CV(%): 6.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Expression of angiopoietin-2 and vascular endothelial growth factor receptor-3 correlates with lymphangiogenesis and angiogenesis and affects survival of oral squamous cell carcinoma." in: PLoS ONE, Vol. 8, Issue 9, pp. e75388, (2013) (PubMed).
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: "Expression of angiopoietin-2 and vascular endothelial growth factor receptor-3 correlates with lymphangiogenesis and angiogenesis and affects survival of oral squamous cell carcinoma." in: PLoS ONE, Vol. 8, Issue 9, pp. e75388, (2013) (PubMed).
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- 抗原 See all FLT4 ELISA试剂盒
- FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))
- 别名
- FLT4 (FLT4 产品)
- 别名
- FLT41 ELISA Kit, LMPH1A ELISA Kit, PCL ELISA Kit, VEGFR3 ELISA Kit, AI323512 ELISA Kit, Chy ELISA Kit, Flt-4 ELISA Kit, VEGFR-3 ELISA Kit, Vegfr3 ELISA Kit, cb1059 ELISA Kit, fms related tyrosine kinase 4 ELISA Kit, FMS-like tyrosine kinase 4 ELISA Kit, fms-related tyrosine kinase 4 ELISA Kit, FLT4 ELISA Kit, Flt4 ELISA Kit, flt4 ELISA Kit
- 背景
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Protein Function: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3- kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr- 185', and of AKT1 at 'Ser-473' (By similarity). .
Background: Fms-related tyrosine kinase 4, also known as FLT4 or VEGFR3, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. FLT4 has an essential role in the development of the embryonic cardiovascular system before the emergence of the lymphatic vessels. It has been found that FLT4, which provides proangiogenic signaling when expressed on endothelium, may also have antiangiogenic properties when expressed at an avascular site by nonendothelial cells. FLT4 is also regarded as a regulator of vascular network formation.
Synonyms: Vascular endothelial growth factor receptor 3,VEGFR-3,2.7.10.1,Fms-like tyrosine kinase 4,FLT-4,Tyrosine-protein kinase receptor FLT4,Flt4,Flt-4, Vegfr3,
Full Gene Name: Vascular endothelial growth factor receptor 3
Cellular Localisation: Cell membrane, Single-pass type I membrane protein . Cytoplasm . Nucleus . Ligand-mediated autophosphorylation leads to rapid internalization.. - 基因ID
- 114110
- UniProt
- Q91ZT1
- 途径
- RTK signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGF Signaling
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