NRP2 ELISA 试剂盒
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- 抗原 See all NRP2 ELISA试剂盒
- NRP2 (Neuropilin 2 (NRP2))
- 抗原表位
- AA 23-857
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10.000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat Neuropilin-2
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: Q23-D857 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: Q23-D857 - Featured
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Neuropilin 2 (NRP2) ELISA Kit
NRP2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Neuropilin 2 (NRP2) ELISA KitNRP2 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Neuropilin 2 (NRP2) ELISA KitNRP2 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neuropilin 2 (NRP2) ELISA KitNRP2 适用: 小鼠 AA 23-863 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the neuropilin family.
Tissue Specificity: Found in certain neuronal populations of the CNS and in other non-neuronal tissues including mesenchymal tissue lining in the ribs.
- 板类型
- Pre-coated
- 实验流程
- rat Neuropilin-2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Neuropilin-2 has been precoated onto 96-well plates. Standards(NSO, Q23-D857) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neuropilin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Neuropilin-2 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat Neuropilin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, cell lysates or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat Neuropilin-2 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.6, Standard deviation: 0.058, CV(%): 3.6
- Sample 2: n=16, Mean(ng/ml): 3.5, Standard deviation: 0.161, CV(%): 4.6
- Sample 3: n=16, Mean(ng/ml): 6.1, Standard deviation: 0.317, CV(%): 5.2,
- Sample 1: n=24, Mean(ng/ml): 1.9, Standard deviation: 0.089, CV(%): 4.7
- Sample 2: n=24, Mean(ng/ml): 4.2, Standard deviation: 0.231, CV(%): 5.5
- Sample 3: n=24, Mean(ng/ml): 7.3, Standard deviation: 0.467, CV(%): 6.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all NRP2 ELISA试剂盒
- NRP2 (Neuropilin 2 (NRP2))
- 别名
- NRP2 (NRP2 产品)
- 别名
- NP2 ELISA Kit, NPN2 ELISA Kit, PRO2714 ELISA Kit, VEGF165R2 ELISA Kit, 1110048P06Rik ELISA Kit, Np-2 ELISA Kit, Np2 ELISA Kit, Npn-2 ELISA Kit, Npn2 ELISA Kit, neuropilin-2 ELISA Kit, neuropilin 2 ELISA Kit, NRP2 ELISA Kit, Nrp2 ELISA Kit
- 背景
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Protein Function: High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.
Background: NRP2(Neuropilin-2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of receptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in Chinese hamster ovary cells. The NRP2 gene is mapped to 2q33.3. The NRP2 gene contains 17 exons and spans about 112 kb. This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with null mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 (PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine number and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, and in NRP2-null brain slices cortical layer V and dentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of NRP2 to apical dendrites and of Npn1 to all dendrites of cortical pyramidal neurons.
Synonyms: Neuropilin-2,Vascular endothelial cell growth factor 165 receptor 2,Nrp2,
Full Gene Name: Neuropilin-2
Cellular Localisation: Membrane, Single-pass type I membrane protein. - 基因ID
- 81527
- UniProt
- O35276
- 途径
- Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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